Expression Of CK14and HPV16E6mRNA In Peripheral Blood Of Patients In Early Stage Cervical Carcinoma And Its Relationship With Cervical Cancer Micrometastases

Objective: Detect the expression of Tissue-specific marker cytokeratin14(CK14) andRelatively specific marker of cervical cancer human papillomavirus16E6(HPV16E6)in patients with cervical cancer,Cervical intraepithelial neoplasia Ⅲ and cervicalinflammation，and explore their correlation with the peripheral blood micrometastasisof cervical cancer to assess the clinical value of detection of micrometastasis,andprovide the theoretical basis for clinical treatment and assessment of prognosis.Methods:The RT-PCR method was used to detecte the expression of CK14mRNAand HPV16E6mRNA in the peripheral blood of50cases of cervical cancer,20casesof Cervical intraepithelial neoplasia Ⅲ,20cases of cervical inflammation patients,and compared the deviation in detection rates about these two proteins in cervicalcancer group and other groups, and compared the expression of CK14mRNA andHPV16E6mRNA in the clinical stage, histological grade and lymph node metastasisof cervical cancer.Results:1. The expression of CK14mRNA in cervical cancer group was significantlydifferent than it in control group,it had some correlation with clinical stages ofcervical squamous cell carcinoma, myometrial invasion and lymph node meta-asis, but it had no relationship with patients’ age and histological grade.(1) The positive expression rates of CK14mRNA in peripheral blood of patients withcervical cancer, Cervical intraepithelial neoplasia Ⅲ and cervical inflammation were24%(12/50),0(0/20),and0(0/20) respectively, the differences among them werestatistically significant (P<0.05). (2) The positive expression rate of CK14mRNA in cervical cancer of clinical stageⅡA was significantly higher than the stage ⅠB(53.3%，11.4%), and the differencebetween them was statistically significant(P＜0.01);The positive expression of CK14mRNA in deep myometrial invasion group was higher than superficial myometrialinvasion group,and the difference was statistically significant(P＜0.05);In addition,theCK14mRNA positive rate in cases with lymph node metastasis was sighificantlyhigher than which without lymph node metastasis(P＜0.001).(3) The positive expression rate of CK14mRNA in peripheral blood of cervicalcancer patients had no relationship with patients’ age and histological grade(P＞0.05).2．The expression of HPV16E6mRNA had no relationship with clinical stages ofcervical squamous cell carcinoma, myometrial invasion, lymph node metastasis,patients’ age and histological grade.(1) The positive expression rates of HPV16E6mRNA in peripheral blood of cervicalcancer, Cervical intraepithelial neoplasia Ⅲ and cervical inflammation were12%(6/50),0(0/20),and0(0/20) respectively, the differences among them were notsignificant (P＞0.05).(2) The positive expression rate of HPV16E6mRNA in cervical cancer of clinicalstage ⅡA was significantly higher than the stage ⅠB(26.7%，5.7%), but thedifference was not significant(P＞0.05);The positive expression of HPV16E6mRNAin cases with lymph node metastasis was higher than which without lymph nodemetastasis(22.2%，9.8%),but the difference was not significant(P＞0.05).(3) The positive expression rate of HPV16E6mRNA in peripheral blood of patientswith cervical cancer had no relationship with patients’ age, histological grade andmyometrial invasion(P＞0.05).3. The expression of CK14and HPV16E6mRNA in peripheral blood of cervicalcancer patients were positively correlated.There are12cases of positive expression of CK14mRNA.In this case,we alsohave4cases of postive expresses of HPV16E6mRNA.CK14may have some positivecorrelation with HPV16E6(γ=0.336，X2=4.406,P＜0.05).Conclusion: The positive expression rate of CK14mRNA in peripheral blood of patients with cervical cancer had some relationship with FIGO stage, myometrialinvasion and lymph node metastasis,but it had no relationship with patients’ age andhistological grade.The positive expression rate of HPV16E6mRNA in peripheralblood of patients with cervical cancer had no relationship with FIGO stage, patients’age,histological grade, myometrial invasion and lymph node metastasis. Theexpression level of CK14mRNA may be associated with development and prognosisof cervical cancer. CK14mRNA in peripheral blood can be used as an importantindicator of predicting recurrence after treatment and predicting disease progressionand prognosis in cervical cancer. The detection rate of HPV16E6mRNA in peripheralblood of patients with cervical cancer was low, its clinical significance remains to bestudied further. Compared the two, CK14mRNA is more likely to be a highsensitivity and specificity indicator of detection of micrometastases in cervical cancer.