| Objective：Through building SDRat pancreatic cancer model to find out potential moleculartargets for Pancreatic Cancer diagnosis and treatment by analyzing and comparing theproteins expressed in SDRats pancreatic cancer tissues and natural pancreatic tissues.Methods:1.establish an animal model with pathological characteristics of human pancreascarcinoma2.Proteomic analysis of SDRats pancreatic cancer tissues and SDRats nanturalpancreatic tissues by two-dimensional gel electrophoresis(2-DE)3.differentially expressed proteins were analyzed and identified by matrix-assistedlaser desorption/ionization time of flight massspectrometry(MALDI-TOF-MS).4.verification Proteomic Peroxiredoxin-1by immunohistochemistry.Results:1.57%(23/40)SD Rat generation pancreatic carcinoma in the model group withpathologically verified2. There were159differentially expressed proteins in the pancreatic cancer tissues.18proteins were successfully identified by MS,in which11proteins were overexpressedin tumors while the other7proteins were underexpressed.3. The increased level of Peroxiredoxin-1protein in SDRat pancreatic cancer wasfurther confirmed by immunohistochemical.Conclusion:1.18differentially expressed proteins were successfully characterized by comparative proteomic analysis in which11proteins were overexpressed in tumors while the other7proteins were underexpressed. These differences can be used as a diagnostic markerproteins for dynamic observation of the development of pancreatic cancer, as a newtarget for gene therapy, and can serve as a marker for evaluation of treatment effect.2.Peroxiredoxin-1protein was highly expressed in pancreatic cancer tissue in rats canbe used as diagnostic markers and therapeutic targets. |
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