Establishment Of Compressed Model Of Cervical PLL In Beagle And The Research Of Sympathetic Nerve Innervation On PLL

Background: Cervical spondylosis is one of the most common clinical disease. Thegrowth of young white-collar crowd, who are under high-load working pressure and deskwork hours excessive, cervical spondylosis has gradually evolved to become a socialdisease, which mainly can be a pain in the neck discomfort, upper limb soreness andnumbness, pain, body paresthesia and gait, limb muscle strength barriers. Generallyspeaking, the diagnosis of cervical spondylosis is not complicated with detailed casehistory inquire, careful physical examination and appropriate imaging examination, whichcan accurately located which nerve was compressed and aim directely decompress it, andget a better effect of treatment.At present, there can be typing cervical spondylosis to four categories in our country:cervical spondylotic myelopathy, cervical spondylotic radiculopathy, vertebral artery typeof cervical spondylosis, and sympathetic cervical spondylosis, where only define twocategories in the western country is that: cervical spondylotic myelopathy, cervicalspondylotic radiculopathy. Vertebral artery type of cervical spondylosis and sympatheticcervical spondylosis turn out similar symptoms contains cervical vertigo, headache,whereas the main different between the two typies is the transverse foramen stenosisand/or hyperplasia osteophyma compressed the former type.Sympathetic cervical spondylosis is a common disease which medical scholars andspine surgeon paid close attention on. During the second cervical spondylosis special topicmeeting, it was defined as follow: degeneration of cervical intervertebral disk stimulate orcompress cervical sympathetic nerves, and course a series of reflectivity symptom. Theclinical manifestation conclude headache, vertigo, tinnitus, tachycardia, obfuscation,flustered and so on, which mainly the sympathetic nervous excitation. According tostatistics, the examination of blood stream in cervical blood vessel, neuropotentical, e.n.tsystem all without disorder, either organic emotionally labile-asthenic disorder orfunctionality of no abnormalities. The unstable of cervical vertebral is one reason ofcoursing sympathetic nerve disorder, but it not the only factor while many patients nothave a unstable cervical. Now we find some patients’ cervical MRI represent degenerationof cervical vertebrae, intervertebral disk compress PLL and spinal slightly.Scholors found sympathetic nerve innervate in spine area, especially in vertebralbodies, intervertebral disc, anterior longitudinal ligament and posterior longitudinalligament. The former study of our National Natural Science Foundation found many sympathetic nerve fibers innervate in posterior longitudinal ligament of New Zealandrabbit with Sucrose-Phosphate-glyoxylic acid (SPG) staining. Some scholars suggestedthat inflammation and other factors that after stimulation or local environmental changescan lead to a proliferation of nerve fibers, nerve fiber expression of the polypeptide is aneffective method to detect the number of nerve fibers.Objective: Under the circumstances of no unified viewpoint with the symptoms of thedisorder of the cervical sympathetic nerve, while the routine anterior cervicaldecompression surgery with the removal of the intervertebral disc and posteriorlongitudinal ligament, and the patients’ sympathetic symptoms can be improved, weassume that whether the longitudinal ligament compressed and such patients a series ofsympathetic nerve activity or inhibit. The nerves were compressed which innervate onPosterior longitudinal ligament change would cause a series of neck discomfort reactions.In this study, we intend to establish an animal model of cervical posterior longitudinalligament under compressed, after fed for several weeks we operate to remove the posteriorlongitudinal ligament. Use special decoration method to observe the morphology of PLL,and measure the change of nerve fibers through NPY between groups.Methods: In disease diagnosis and research of the nervous tissue, the application ofconventional HE staining can be observed in neurons and nerve fibers in the whole picture,but their fine structure and some special ingredients require special staining method todisplay. Although it has long been described cervical posterior longitudinal ligament of thedistribution of sympathetic fibers, but the detection of sympathetic neurotransmitter releaseby detecting sympathetic to determine the positioning of the local tissue. Sympathetic areadrenergic nerves, it can release of monoamine neurotransmitters, so you can use themethod of the reality of monoamine neurotransmitters of the sympathetic nerve fibersinnervate on C4/5PLL. Neuropeptide Y may be the expression of sympathetic nerve fibers,we can be with the neuropeptide Y staining morphology of the sympathetic nerve fibers ofthe posterior longitudinal ligament. Radioimmunoassay (RIA) determination of ultramicroamounts to a combination of in vitro specificity of radioisotope measurement of highsensitivity, accuracy and antigen-antibody reaction (10-9~10-15g) the substance of the newtechnology.In our former study, we found sympathetic nerve fibers innervate on cervical PLL.This time, We distributed group as experimental group and sham operation control group,and operated anterior cervical discectomy fusion (ACDF) surgery, with the aim of establishing model of cervical intervertebral disc compressed cervical PLL, while the shamoperation control group operated exposure process, reserve integrality of cervicalintervertebral disc and maintains savagery of spinal canal, this is the step one. The step twois observe the sympathetic nerve innervation after chamisty decoration with four beagleschoosed random, and the step three is quantified the change of the two groups useradioimmunoassay method with the surplus beagles.Result: The result found three significant points, one is the beagle is fit for establishinganimal model in the cervical area, two is that both groups of cervical PLL collectionscontain sympathetic nerve fibers, and three is the compressed group had a higherpresentation than sham operation control group under radioactivity immunizationmeasurement, the compressed group’ NPY as follow:61.78pg/100μl,73.22pg/100μl,63.67pg/100μl,79.78pg/100μl）,65.33pg/100μl, and68.81pg/100μl., and sham operated group’NPY as follow：34.31pg/100μl,42.91pg/100μl,35.23pg/100μl,39.72pg/100μl,36.41pg/100μl,40.62pg/100μl.Conclusion: In summary, we establish the model of cervical slipped disc compress PLL,regared Beagles easy to clear anatomical structure of anterior cervical surgery, goodtolerance surgery operation, rigid fixation of the vertebral easily obtained and found plentyof sympathetic nerve fiber in cervical PLL, after what we can research the path betweencervical PLL and sympathetic trunk in the next study.