| In this study, extraction, separation, antioxidant activity and mass spectrometryinformations have been applied successfully to investigation of rapeseed, Garciniamangostana L.and secondary metabolites from the plant endophytic fungusPestalotiopsis fici.Rape (Brassica napus L.) is a cruciferous plant. Rapeseed oil, with a relativelyhigh amount of unsaturated fatty acids, is significant for human health, such asreducing total cholesterol and blood pressure. High content of unsaturated fatty acidshas obvious antioxidant activity. Due to the advantages of the high content ofrapeseed oil and vast planting areas, it has received extensive attention and research.Application of a green, simple technique for extracting rapeseed oil from rapeseed willbe the trend of development in the future. A lot of rapeseed meals were thrown awayas a waste after the extraction of rapeseed oil from the rapeseed. tannins(proanthocyanidins) in the meal have a strong antioxidant and free radical scavengingactivity. Application of accelerated solvent extraction technology has a prospect toextract procyanidins from rapeseed meal. In this study, the main rapeseed oilextracted from rapeseed using two extraction methods–Soxhlet and SFE-CO2extraction–has been successfully achieved. Taking the aspects of residual solvent,reaction time, solvent consumption and GC-MS analysis into account, it shows thatSFE-CO2process is an effective method for extracting rapeseed oil. The RSM wasapplied to optimize the extraction process, the optimal experimental conditions were asfollows: extraction temperature40℃, extraction pressure345bar, extraction time3hand the particle size of sample60mesh, the yield of rapeseed oil is32.65±1.01%. Accelerated solvent extraction and ultrasonic extraction were employed to extractproanthocyanidins from rapeseed meal. Compared with ultrasonic extraction method,ASE is time-saving, convenient, high efficiency and is suitable for extraction ofproanthocyanidins from rapeseed meal. The best extraction conditions of acceleratedsolvent extraction determined by response surface were as follows: concentration ofethanol60%, extraction temperature60℃, cycle number3times.Under optimumconditions, the content of procyanidins was7.370mg/g, but the content ofprocyanidins using the ultrasonic extraction was less than7.000mg/g. Acceleratedsolvent extraction of procyanidins from rapeseed meal provides a simple, rapidanalytical methods.Garcinia mangostana L. is a tropical evergreen tree that belongs to the familyGuttiferae. Mangosteen is known as one of the best natural sources of xanthones.Xanthones have been reported to have high pharmacological and biological activity.Rapid extraction and separation of its active ingredients have a certain practicalsignificance. Active xanthones in the Garcinia mangostana L. was extracted bysupercritical fluid extraction technology with alcohol as entrainer, and two xanthones,α-Mangostin and Gartanin, were isolated from the crude extract through the device ofrapid preparative chromatography and identified by mass spectrometry. The purity oftwo isolated compounds was higher than90%via detection of HPLC-DAD.Meanwhile, the fragmentation mechanism of two compounds were studied throughelectrospray ionization tandem mass spectrometry (ESI-MSn). Compared the peakarea of the sample after joining DPPH, scavenging DPPH effect of Gartanin andα-Mangostin is respectively90.67%,38.17%. Two xanthones have a certainantioxidant activity through the analysis of HPLC-MS. Supercritical fluidextraction-Rapid preparative chromatography-mass spectrometry provides a simple,fast and effective way for extraction isolation, identification of the chemical constituents in the Garcinia mangostana L.The species of the fungal genus Pestalotiopsis are widely distributed in variousecosystems. In recent years, many of the new structure and good activity ofsecondary metabolites from the plant endophytic fungus Pestalotiopsis fici. Thesesecondary metabolites have better activities with anti-tumor, antibacterial, antioxidantactivity. There secondary metabolites(Pestalofone D,Pestalofone E and Pestalofone F)from the plant endophytic fungus Pestalotiopsis fici. were studied using the technologyof mass spectrometry. Through the characteristic fragment ions of there secondarymetabolites, mass spectrometry can quickly distinguish and analyze the secondarymetabolites. Secondly, technology of high performance liquid chromatographic-massspectrometry was applied into studying their antioxidant activities. There secondarymetabolites’ scavenging DPPH effect is respectively12.66%,20.97%,14.66%. Theresearch showed that there secondary metabolites had some antioxidant activity. |
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