| 1ObjectiveCombined with the incidence of chronic hepatitis B in China, the current level of health care in China and China’s current economic realities, according to the "kidney-volt gas theory, on the basis of preliminary studies, kidney disinfection of CHB patientsHBV transgenic mice role as one of the research through clinical, laboratory co attempt to explore the Chinese medicine treatment of CHB patients and HBV transgenic mice efficacy, better for human health, relieve the suffering of the CHB patients, improve their quality of life, but alsoprovide a better foundation for future experimental study of HBV clinical co.2Methods2.1The kidney Qingdu retrospective study the clinical efficacy of the CHB patientsThe past two years (January2010, January2012) by reviewing the mentor ProfessorShen Qiang liver disease outpatient Affiliated Hospital)(Guangzhou University ofTraditional Chinese Medicine diagnosis of patients with chronic hepatitis B cases, in line with the inclusion criteria of this study, a total of31cases, according to the case during treatment of kidney disinfection treatment, will be divided into two groups conducted aretrospective observation. Treatment group (kidney disinfection method) of17cases,14cases in the control group (a nucleoside analogue antiviral). Retrospective analysisbefore treatment, after treatment serological indicators (including liver function), virological indicators change. Prepared by the Ministry of Health Organization expertsrefer to2005, published by the China Press of Traditional Chinese Medicine Traditional Chinese Drug Guidelines for clinical research (Trial)", assessed the symptoms were improved. Reference to the Chinese Medical Association of Hepatology, Infectious Diseases credit enacted in2005,"Chronic hepatitis B prevention and treatment guidelines, the development of hepatitis B virus to improve the efficacy of standard andassessed.2.2The kidney Qingdu of HBV transgenic mice the role of observationTake the HBV transgenic mice,50male and female, were randomly divided into five groups, the model control group8; administration of four groups: the lamivudine group9; kidney disinfection method the high dose group, make updose group in the kidney disinfection method, kidney, disinfection method, low-dose group,11in each group. Normal mice, the blank control group,10. Mice were weighed once a week. The day prior to admini stration to mice eyeballs to take blood using quantitative PCR detection of serum HBV-DNA content, followed by daily to give the corresponding drug by group, by gavage,40days later, again the eye blood using quantitative PCR method to detect serum HBV-DNA content, the virus observed after treatment to improve the situation, and finally al1the mice were sacrificed to collect liver tissue, pathological changes in liver were observed.3Results3.1The kidney Qingdu clinical efficacy in CHB patientsThe treatment group (kidney disinfection method) and the control group (a nucleoside analogue antiviral drugs) before treatment, symptom score, the difference was notsigni ficant (P>0.05). Group after treatment than before treatment of the symptom scoredecreased significantly (P<0.05); the control group were abdomi nal nausea, abdominal distension, loss of appetite, dry mouth waiting for symptom score before treatment were significantly decreased (P <0.05), symptom score before and after treatment showed nosigni ficant significance (P>0.05). Between the two groups after treatment, the differences were significant (P<0.05), suggesting that the treatment group than the control group in improving symptom scores. Clinical efficacy, the treatment group haveefficiency and total have Comparison of efficiency and the control group’s, withoutsignificantly with sexual significance (P>0.05); Comparison of the control group without the efficiency and the treatment group of, non-significant with sexual significance (P>0.05), prompted the kidney disinfection method in the antiviral efficacy similar to thenuclcoside analogue antiviral.3.2The kidney Qingdu of CHB patients with liver functionTreatment group (kidney disinfection method) and the control group (a nucleoside analogue antiviral) to restore liver function in ALT and AST in the A/G, r-the GT and other aspects are better, statistical analysis, the treatment of ALTThe recovery rate was higher (P<0.05) difference I had no obvious significant (P>0.05).3.3kidney toxic method of CHB patients with serum markers and HBV-DNA inNo significant treatment group (kidney disinfection method) and the control group(nucleoside analogue antiviral drugs) of HBV-DNA negative conversion rate of the overall difference in significance (P>0.05); of HBsAg, HBeAg negative conversion rate of the two groups overallThe differences were not significant (P>0.05). HBV-DNA values in each group before treatment was no significant difference (P>0.05), no statistically significant, with better comparability.Serum HBV-DNA in the control group after treatment than before treatment on the values fell slightly, but the difference was significant (P>0.05). After treatment, serum HBV-DNA values than before treatment fell slightly, but the difference was no significant (P>0.05). After treatment, the treatment group and control group, serum HBV-DNA numeric comparison, the difference was notsignificant (P>0.05).3.4The kidney Qingdu method HBV Transgenic mice serum HBV-DNALow dose group of kidney clear drug dose group in the kidney disinfection method, kidney disinfection method, high-dose group, lamivudine tablet group before treatment, HBV-DNA in the numerical comparison, the difference was not significant (P>0.05), statistically significant, with better comparability. Dose group, model control group, kidney disinfection method, low-dose group, the kidney disinfection method in each group before and after treatment serum HBV-DNA numeric comparison, the difference was not significantly significant (P>0.05). High dose group of kidney di sinfectionmethod, the logarithm of serum HBV-DNA before and after lamivudine treatment ofchipsets, the difference was significant (P<0.05). Lamivudine group after treatment with the model control group, serum HBV-DNA on the numerical comparison, the difference was significant (P<0.05); kidney after treatment disinfect i on law low-dose group, kidneydisinfection method in the dose group kidney disinfection method of high-dose group and model control group scrum HBV-DNA value differences were not significantsignificance (P>0.05). Dose group after treatment, lamivudine group, kidney disinfectionmethod low-dose group, kidney disinfection method, kidney clear toxic high-dose group, serum HBV-DNA between the numerical comparison, the difference was not significantsignificance (P>0.05).3.5The kidney Qingdu transferred mice liver histopathology of HBVModel control group of transgenic mice liver pathological changes in the dense infiltration of lymphocytes:expansion of hepatic venous congestion, portal area, mild proliferation of fibrous tissue. Performance of similar clinical mild hepatitis. With increasing concentrat ion of bushen disinfection method, the inflammation to ease the liver plate of the high dose group arranged in neat rows, reduce the lymphocyte infiltration. Were seen scattered in the lamivudine therapy mouse liver periportal lymphocyte infiltration, fibrous tissue hyperplasia, with mild fatty degeneration of liver cells around the central vein. Therefore, the traditional Chinese medicine to improve the inflammatory symptoms of the liver tissue of transgenic mice is better, and lamivudine in liver tissue of transgenic mice there is mild steatosis, may be due to the toxicity of the drugs on liver cell metabolism, or pathological liver injury in transgenic mice appear to change this result to be confirmed by further studies.4Conclusions4.1CHB patients to improve symptoms better than a nucleoside analogue antiviral drugtreatment of kidney clear.4.2CHB patients from the drug treatment of kidney clear, ALT improvement was also superior to the antiviral nucleoside analogues.4.3Kidney disinfection antiviral efficacy similar to the nucleoside analogue antiviral.4.4the advantages of traditional Chinese medicine treatment of CHB is to improve symptoms and ALT.4.5Kidney disinfection method for HBV Transgenic mice serum HBV-DNA, the higher the dose, the effect is more obvious.4.6Chinese medicine transgenic mouse liver tissue inflammatory symptoms improve. |
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