A Study On The Relationship Between The OX40/OX40L Molecules And The Pathogenesis Of SLE

Objective:To detect the OX40and OX40L on peripheral blood cells in SLE patients andelaborate their roles in the pathogenesis of SLE and clinial practice.Methods:62patients with SLE as defined by the American College of RheumtologyCriteria and40healthy age and sex matched controls were included into the study.Collectclinical and related laboratory materials.They were divided into active group(≥10)42casesand inactive group(0～9)according to the SLEDAI system.They were also divided intolupus nephritis group25cases and without lupus nephritis group37cases according torenal involvement.Use flow cytometry to detect the expression rate of OX40and OX40Lon peripheral blood cells.A3months follow up recorded the materials of the activegroup.Twelve cases were collected.Detect the OX40and OX40L expression rate beforeand after therapy.Compare the OX40and OX40L expression rate among the SLE groupand helthy controls、lupus nephritis and without lupus nephritis groups、active gruop andinactive group、before and after therapy group.Results:1.Compared with healthy controls,the expression rate of OX40on CD4+T cellsin SLE patients group was significantly increased.Whereas no significant difference wasfound on CD8+T cells.2. Compared with healthy controls，the expression rate of OX40Lon B cells、NK cells、monocytes in SLE patients group were significantly increased.3.Compared with without lupus nephritis group, the expression rate of OX40on CD4+Tcells(P﹤0.0001)and OX40L(P﹤0.05) on B lymphocytes in lupus nephritis patients weregreatly upregulated.whereas the OX40L on NK cells、monocytes were no difference.4.Compared with inactive group, the expression rate of OX40on CD4+T cells (P﹤0.0001)and OX40L(P﹤0.05) on B lymphocytes in lupus nephritis patients were greatly upregulated.whereas the OX40L on NK cells、monocytes were no difference.5.Among6cases with less effective treatment, the expression rate of OX40on CD4+T cells andOX40L on B lymphocytes were no difference（P﹥0.05）between before and aftertreatment.On the contrary,among another6cases with effective treatment, the expressionrate of OX40on CD4+T cells and OX40L on B lymphocytes was greatly reduced(P﹤0.05).Conclusion:1.The number of OX40+CD4+T lymphocytes subsets、 OX40L+Blymphocytes、 OX40L+NK cells、 OX40L+monocytes in SLE patients group weresinificantly increased.2.The expression of OX40on CD4+T lymphocytes and the expression of OX40L on Blymphocytes were greatly upregulated among the active and lupus nephritis patientsgroups.3.As the conditions improved, the expression of OX40on CD4+T lymphocytes andthe expression of OX40L on B lymphocytes were greatly reduced.4.OX40/OX40L moleculars may play important roles in the pathogenesis of SLE.