Study On Gold Nanoparticles Modified Glassy Carbon Electrode And Its Applications In Detection Of Epirubicin

In this thesis, a glassy carbon electrode was chemically modified with goldnanoparticles and applied to the detection of epirubicin. Some important results wereobtained as the followings.Gold nanoparticles were prepared by directly chemical reduction of sodiumcitrate to gold chloride acid with different particle sizes. Characterizations of theprepared gold nanoparticles indicate that these particles have the regular shapes,uniform particle sizes and good dispersion in solution.A glassy carbon electrode as a basic electrode was firstly modified by theelectrochemical polymerization of o-phenylenediamine, the different sizes goldnanoparticles were secondly adsorbed on the layer of poly-o-phenylenediaminethrough the electrostatic interactions between amino groups in thepoly-o-phenylenediamine and nanoparticles. Characterizations of the modifiedelectrode shows that the gold nanoparticles are uniformly distributed on the electrodesurface, the distributions of small size gold nanoparticles are closer from each otherand denser than those with larger sizes.The electrochemical behavior of epirubicin at the gold nanoparticles modifiedglassy carbon electrode was studied by cyclic voltammetry and differential pulsevoltammetry. Under the optimal experimental conditions of0.01000mol/L HClsolution, quiet time of480.0s, epirubicin shows a sensitive reduction peak at-0.3300V.The reduction peak current is proportional to the epirubicin concentration in the rangeof3.450×10^-9mol/L¹.725×10^-7mol/L with R=-0.9994, SD=5.047×10^-9μA, and thedetection limit of3.450×10^-9mol/L. The method can be used in the detection ofepirubicin with high sensitivity, simple operation, small amount of sample, and offersa new approach for the electrochemical detection of epirubicin.