Effects Of Ligustrazine On Expression Of FoxO3a、Mafbx And MuRF1in Denervated Skeletal Muscle Atrophy Rats

Objective:To investigate the effects of ligustrazine on expression of FoXO3a、MAFbx and MuRF1in denervated skeletal muscle atrophy rats.Methods:54female Wistar rats were randomly divided into3groups, which included normal control group (A n=6), denervated control group (B n=24), ligustrazine intervention group (C n=24).The denervated control groups and ligustrazine intervention groups were subjected to establishment of denervated gastrocnemius models.Normal control group (0day) without any treatment; ligustrazine intervention groups were injected with ligustrazine injection80mg/kg·d by intraperitoneal injection method; the denervated control groups were intraperitoneally given the same dose of saline.One group rats were sacrificed at day2,7,14,28after denervation and both side of gastrocnemius muscle were removed and muscle wet weight was weighed.FoXO3a, MAFbx and MuRF1mRNA and protein expression levels at each time were detected by RT-PCR and Western Blot test. Statistical software was used to analyze the effects of ligustrazine on expression of FoXO3a、MAFbx and MuRF1Results:The wet muscle ratio in the ligustrazine intervention group were significantly higher than the denervated control group (p<0.05).FoXO3a, MAFbx and MuRF1mRNA and protein expression in the ligustrazine intervention group were lower than the denervated control group (p<0.05).Conclusion:Ligustrazine may by reducing FoXO3a、MAFbx and MuRF1mRNA and protein expression to delay denervated skeletal muscle atrophy.