Study On The Mechanism Of TLR3 In HBV Intrauterine Infection Of Babies Whose Mother Are HBsAg Positive

OBJECTIVE:①To investigate the relationship between TLR3 in the PBMC of the unbilicalvein blood of babies whose mothers are HBsAg posotive and HBV intrauterineinfection.②To explore the relationship between immunocytes concerning PBMC and theHBV intrauterine infection.③To explore the mechanism of TLR3 in HBV intrauterine infection .METHODS:①The epidemiological base line data and umbilical vein blood of 52HBsAg-positive pregnant women were collected from Taiyuan infectious hospitalfrom July 2011 to January 2012.②Experiment was performed in vitro: The PBMC were separated from umbilicalvein blood and then were cultured. The PBMC of HBV intrauterine infection wereintervented by HBV+PolyI:C and HBV.The PBMC with negative HBV intrauterineinfection were intervented only by HBV.③The protein expession of TLR3 and the percentages of B-cells,T-cells,DC andTreg were detected by FCM.RESULTS:①The expression level of TLR3 protein was higher in PBMC with negativeHBV intrauterine infection than that in PBMC with positive HBV intrauterineinfection(32.18±11.36,30.29±11.04),but no significant difference was observed（t=0.410，P=0.684）.After the intervention of PolyI:C, the expression level of TLR3increased,but still no significant difference was observed (32.18±11.36, 32.16±9.40;t=-1.824,P=0.078).②Proportion of B-cells with negative HBV intrauterine uninfection was higherthan the ones with positive HBV intrauterine infection(7.38±6.40;6.76±4.01),but nosignificant difference was observed (t=0.210,P=0.834).After the intervention ofPolyI:C, percentage of B-cells with positive HBV intrauterine uninfection decreased,but still no significant difference was observed(7.38±6.40,5.14±2.64;t=0.768,P=0.447). ③Percentage of CD4+T-cells with negative HBV intrauterine infection washigher than the ones with positive HBV intrauterine infection,but no significantdifference was observed(41.14±20.08,33.82±2.60;t=0.618,P=0.543),while percentageof CD8+T-cells with negative HBV intrauterine infection was higher than the oneswith positive HBV intrauterine infection(11.30±5.87,11.14±9.64) with no significantdifference(11.30±5.87,11.14±9.64;t=0.041,P=0.968).The difference of ratio ofCD4+T-cells to CD8+T-cells between negative and positive HBV intrauterine infectiongroups was not significant(4.30±2.28,4.75±3.23;t=-0.30,P=0.767).④Percentage of mDC with negaitive HBV intrauterine infection was higher thanthe one with positive HBV intrauterine infection(0.84±0.58,0.67±0.34) but with nosignificant difference (t=-0.485,P=0.633).While percentage of pDC with negaitiveHBV intrauterine infection was higher than the one with positive HBV intrauterineinfection,but with no significant difference there was(0.92±0.91,0.91±0.46;t=0.020,P=0.984).⑤Percentage of CD4+CD25+Treg with negative HBV intrauterine infection washigher than the one with positive HBV intrauterine infection(4.69±3.41,3.00),but withno significant difference between them(t=0.474,P=0.646).CONCLUSIONS:①The fact that expression level of TLR3 protein with negative HBVintrauterine infection was higher than the one with HBV intrauterine infection andthat intervention of PolyI:C caused the increase of the expression level of TLR3protein indicates that HBV intrauterine infection may be linked to TLR3 proteinexpresson.②The fact that the percentages of B-cells,CD4+T-cells and mDC with negativeHBV intrauterine infection were higher than the ones with positive intrauterineinfection indicates that the B-cells,CD4+T-cells,mDC may be a protective factoragainst placenta HBV infection.③The fact that association between percentage of CD8+T-cells,pDC as well asCD4+CD25+Treg and the CD4+/CD8+T-cells was not discovered indicates that furtherstudy is required.