Effects Of AS2O3on Apoptosis And Cytokine Release Of Peripheral Blood T Lymphocyte In Sle

Objective:To investigate the apoptosis-induced effect and secretary function on interferon-y(IFN-γ),Interleukin-6(IL-6),Interleukin-10(IL-10)of AS2O3on peripheral blood T lymphocyte in Systemic lupus erythematosus(SLE) patients,which can provide relevant theoretical basis for AS2O3to the clinical treatment of SLE.Methods:Collecting and isolating peripheral blood T lymphocyte with nylon wool in15SLE patients and normal subjects.The purity and cell proliferation of T lymphocytes were determined by flow cytometry(FCM)and trypan blue exclusion.After additing500IU/ml interleukin-2(IL-2),Cultivate it48h.Then with cyclophosphamide(CTX) and different concentrations of AS2O3treated12h and24h,the apoptosis rate of T lymphocytes was analyzed by flow cytometry(FCM).The apoptosis pictures were taken by acridine orange(AO)and ethidum bromide (EB).The contents of IFN-γIL-10and IL-6in T lymphocytes were measured by ELISA.Result:1. In AS2O3groups,the apoptosis of peripheral blood T lymphocyte in SLE patients and normal subjects are creasing along with thg drug concentration and the role of time,which is in time and dose dependence. Compared with normal subjects,the apoptosis rate of SLE patients were different (P<0.05);In CTX groups,the apoptosis of peripheral blood T lymphocyte are also creasing,but it has no significant differences between SLE group and normal subjects(P>0.05).Compared with CTX,AS2O3had more significant effect on the apoptosis of peripheral blood T lymphocyte in SLE patients (P<0.05).2. After AO/EB stained,the apoptosis of T cells indict cellular nucleus reduced and stained in orange in luorescent microscope examination;The dead cellular nucleus were also orange stained but did not shrink,and normal cells were only stained green.3. Compared with normal subjects,the secretion of IFN-y,IL-6,IL-10in SLE group were higher after cultivated in vitro for12h,which have difference in statistics(P<0.05);After intervented respectively by0.8μg/ml AS2O3and160mg/ml CTX after cultivated in vitro for12h in SLE group,the secretion of IFN-γ,IL-6,IL-10reduced comparing to that before intervented,which have statistical significance.Conclusion:1. Compared with normal subjects,the secretion of IFN-γ,IL-6, IL-10and the ratio of IL-10/IFN-γ in SLE group were higher than the normal subjects,which may appear Th2migration.2. Both of AS2O3and CTX can promote the apoptosis of T lymphocyte and inhibite the secretion of IFN-γ,IL-6, IL-10in SLE patients,which may point out the mechanism to treatment of SLE.