| Objective Although,many studies have found that exercise training can promote the restoration of functions of patients with cerebral infarction,the study of training intensity has become more and more important.How long the rehabilitation therapy period should be to gain the best results for functional restoration and whether the intensive training(increase recovery time)can improve clinical outcomes haven’t been confirmed.We observe the expression of GFAP and bFGF in cerebrum after cerebral ischemia-reperfusion in rats by training at different intensities,in order to investigate the possible mechanism of intensive training on improving recovery of motor function after cerebral ischemia-reperfusion in rats.Methods To establish rats model,intraluminal thread method was applied to make the left middle cerebral artery occlusion for2hours and then reperfusion for3,7and14days.After the ischemia-reperfusion model were established,one’hundred and eighty male Wistar rats were randomly divided into training group1-4,control group and sham group.Rats in training group1were trained by swimming once a day for five minitues;Rats in training group2swimming twice a day for five minitues each time.Rats in training group3swimming once a day for ten minitues,and group4were trained twice a day for ten minitues each time,while no training in control group and sham group.The neurological function was evaluated by Bederson’s test,the cerebral infarction volume calculated by tetrazolium chloride(TTC)staining.The apoptosis in peri-infarct cortex were evaluated by TUNEL stain at the3rd,7th,14th days after swimming training.The expression of GFAP and bFGF in the peri-cerebral infracted area were detected using immunohistochemistry.Results There was significant difference between the control group and sham operative group.The Bederson’s evaluation score of the exercise training groups on the7th and14th days were lower than that of the control group.The infarction volume of each exercise training group were significantly lower than those in the control group,the number of apoptosis is reduced,and the rates of positive cell of GFAP and bFGF in all training groups were higher than that of the controls at the3th,7th and14th day after MCAO,especially the training group4.Conclusion Exercise training,especially intensive exercise training,can reduce the induction of apoptosis by cerebral ischemia-reperfusion in rats and adjust the expression of GFAP and bFGF,and then promote brain damage repair and remodeling after cerebral ischemia-reperfusion. |
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