Distribution And Pathogenicity To Identification Of Genes Among Food Samples

Recently, the food security has occurred frequently vicious in the world, whether developed or developing countries, food-borne disease incidence remains high. Foodborne diseases and food contamination and pose a huge growing public health problem worldwide. Staphylococcus aureus (abbreviated S. aureus) is widely present in the natural environment in a variety of physical and chemical factors on the higher resistance, not only of food poisoning caused by important bacterial pathogens, but also the hospital’s main pathogens of clinical infection. From public health point of view, S. aureus has been attracted much attention. Many experts and scholars carried out studies on the biological characteristics of Staphylococcus aureus, detection technology, disease genes and their virulence factors, epidemiological investigation.The detection of Staphylococcus aureus is primarily methicillin-resistant Staphylococcus aureus detection and production of pathogenic toxin of Staphylococcus aureus detection. On the production of pathogenic toxin of Staphylococcus aureus detection by coagulase test and thermostable nuclease activity in experimental testing, for methicillin-resistant Staphylococcus aureus was detected mainly by Kerby - Bauer paper. The experimental design of synthesis of Staphylococcus aureus nuC, caL, pvL pathogenic gene primers to laboratory,685 samples of Staphylococcus aureus as the research object, PCR identification, in order to study the three kinds of Staphylococcus aureus virulence gene distributionand.studied the distribution the virulence genes in Staphylococcus aureus isolates.In the isolation of 221 Staphylococcus aureus, the 91.98% with nuC genes, 24.43% with pvL genes, 98.64% with caL genes. The study indicated that Staphylococcus aureus separated has high probability of carrying toxin.Test of pvL gene in human septic tissue detection rate reached 36.17%, indicating that the pvL gene of Staphylococcus aureus mainly exists in abscess tissue isolation of Staphylococcus aureus in. Living environment is found to contain pvL gene of Staphylococcus aureus accounted for 25.92%, should pay attention to. PvL production by Staphylococcus aureus in a community have the epidemic reports, and health in populations also have a certain proportion of carriers. There is no food in the separation of Staphylococcus aureus pvL gene detection reports, the experiments on 145strains isolated from food in Staphylococcus aureus were detected,28strains contain pvL gene, the detection rate is 19.31%. PvL containing Staphylococcus aureus in raw milk, meat products, frozen food, these two processing of food and temperature, health, population mobility related. Because pvL containing Staphylococcus aureus in healthy population in the carrying rate is very high, dairy and meat processing workshop of large population mobility is more likely to cause the transmission of germs, concluded that the food the middle pvL of Staphylococcus aureus is a man carrying induced spreading.Based on the pvL gene of mouse virulence experiments, injection containing pvL gene of Sa mice die in a short time, mortality is 100%. According to the experimental results to infer virulence containing pvL gene of Staphylococcus aureus with strong pathogenicity, should strengthen pair of pvL gene detection, especially to strengthen from the food source of Staphylococcus aureus in the detection of pvL gene.The pollution situation and epidemiologic conditions of Staphylococcus aureus in crowd, livestocks and food should be elucidated in further research, which will provide basis for the supervision of Staphylococcus aureus, the detection of explosion and spread as well as tracking pollution sources.