Molecular Mechanisms Of Electroacupuncture On Neuropathic Pain In Diabetic Rats

Objective:To study the mechanism of DPNP (diabetic peripheral neuropathy pain，DPNP). Toobserve the behavior changes of the DPNP rats before and after the electro-acupuncturetreatment and to explore its mechanism.Method:1DPNP rat model. female rats accepted intraperitoneal injection of60mg/kgstreptozotocin (STZ) after being fasted10hours before modeling, modeling1234681012weeks to measure their weight, fasting blood glucose and the mechanical threshold atthe bottom of foot.two weeks after modeling, the rats with fasting bloodglucose>16.7mmol/L, the threshold decreased two or more compared to baseline valueswere enrolled.2, The models were randomly divided into control group, EA no1group, EA no2group, sham EA group. The EA no1group received a half-hourlong Zusanlielectroacupuncture stimulation with alternating4/12low-frequency and frequency,intensity1MA, then the EA no2group received Gaohuangshu acupoint electricalstimulation with the same frequency, intensity, duration. Sham EA group received Zusanlihalf-hour acupuncture without electrical stimulation. The control group did not receive anytreatment. To obtain the30min,60min,120min,240min foot mechanical thresholds ofcontrol group, EA no1group, EA no2group, sham EA group after the first and the secondtreatment. to measure the mechanical thresholds at the same time with the former isequivalent to120min time point till its effection disappeared.3, the western blot.To compare the experimental rats and normal control group ratsspinal ganglia expression of nuclear factor kappa B (nuclear factor kappa B, NF-κB) in themolecular level.Results: 1the weight and the echanical threshold of rats these with STZ injection,wassignificantly lower than the rats without STZ injection, then the fasting plasma glucose wassignificantly higher.the mechanical threshold of the the STZ rats shows a clear downwardtrend at1week,a plateau at2-6weeks,a rise at8week,and the disappearment of allodynicphenomenon at12week.2the mechanical threshold of EA no1group was significantly increased. after thefirst day of the EA, the mechanical threshold of no1EA group appears shows a rise at30min,a peak at60min then a downward trend at120min. the Analgesic effect of electricneedle treatment can last up to4days. the mechanical threshold of the rats which acceptedthe second EA treatment shows the platform at30min and no clear downward trend.again aweek after a group of EA electro-acupuncture treatment of plantarmechanical thresholdthan the control group was significantly increased.3the NF-κB molecule expression level of the DPNP rats was significantly increasedcompareing with normal control group.then the expression level of NF-κB in EA groupDPNP rat dorsal root ganglion were markedly lower.Conclusion:1SD rats2weeks after the injection of STZ can reach the standards of DPNP rats,and good stability in the model in2-6weeks.2,4/12low-frequency and frequency of alternating strength1MA long half-hour ofZusanli electroacupuncture treatment has a significant analgesic effect, and the analgesiceffec can last up to4days.multiple EA treatment can enhance the duration of analgesia3, DPNP, in the rat pain molecular substances in the participation of NF-κB.theNF-κB in the regulation of rat spinalganglion may be the target of the electro-acupuncturetreatment.