Interaction Between Molecules Or Iron And Human Serum Albumin As Well As The Investigation Of Fluorescence Carbon Nanoparticles

Human serum albumin (HSA) is the most abundant protein in the plasma, compared with the other proteins, HSA posses some characteristics, such as small weight, lager solubility in water, negative charge, good stability and affinity. Furthermore, HSA can combine with a variety of chemicals, such as metal ions, fatty acids, amino acids, hormones, drugs and so on. By studying the interaction between the chemicals and HSA, the important information of some substances in vivo transit, distribution and metabolic processes can be obtained. Therefore, the studies on interaction between metal ions or organic molecules and HSA play an important role in life sciences, chemistry and clinical.The properties of fluorescent carbon nanoparticles (CNPs) were studied except the interaction between chemicals and HSA in this paper. Because carbon is the most abundant element in nature and low toxic, carbon nanomaterials have been the frontier of technological innovation. On the basis of previous research, three works were carried out, which were listed as follows:1. The interaction between malachite green and HSA has been investigated under the physiological conditions. The fluorescence spectroscopy, circular dichroism and Fourier transform infrared (FT-IR) spectroscopy were performed to determine the binding constants of interaction, the main force and the change of protein secondary structure caused by malachite green. Molecular modeling revealed the main force between them. The experimental results showed that the quenching mechanism between malachite green and HSA was static quenching. The binding constants between malachite green and HSA was2.9×104L/mol and the number of binding sites was1.2.2. The interaction between Mo (Ⅵ) and HSA was carried out under the physiological conditions by using fluorescence spectroscopy and UV spectroscopy. The experimental results showed that the quenching mechanism between Mo (VI) and HSA was static quenching. The binding constant between Mo (Ⅵ) and HSA was1.49×104Lmol-1and the number of binding sites was1.01. 3. CNPs which possess good water-soluble in aqueous solution were synthesized by nitric acid based on oxidizing kerosene soot. The CNPs could be stable for more than three months in water without the reduction of fluorescence intensity. The scanning electron microscopy (SEM) of CNPs showed that the size of CNPs was about50nm. In order to improve properties of CNPs, they were modified by thiocarbamide (TC). The results showed that the fluorescence properties of CNPs were changed. Furthermore, the responses to pH of thiocarbamide-modified CNPs (CNPs-TC) were different from the CNPs. In addition, the effects of adsorption on the CNPs and CNPs-TC with metal ions were investigated, and the efficiency and velocity of adsorption of CNPs-TC was much better than that of CNPs.