Study On Anti-tumor Effect Of Extractions From Gekko Japonicus

We separated extractions from Gekko japonicus in this study. Theirantiproliferative activities and mechanism were evaluated using several cancer celllines in vitro, including esophageal cancer cell lines CaEs-17and hepatocellularcarcinoma cell line QGY-7701. The effect in life span of H₂₂and EAC ascites tumormice, and tumor growth of S₁₈₀and H₂₂tumor-bearing mice in vivo were investigated.At first, Gekko japonicus60%alcohol extracts I, alkaloid-like substance II wereseparated, and analyzed with color reaction which confirm extracts II containalkaloid-like compounds. We also separated extracts A and B from substance II. Bothof them have color reaction, and HPLC assay were used to analyze the result of theseparation.Then, the inhibition of cell growth of extracts I, II on CaEs-17and QGY-7701cells were detected by MTT assay in vitro. The result suggested that alcohol extracts I,alkaloid-like substance II can significantly inhibit hepatocellular carcinoma cell lineQGY-7701and esophageal cancer cell lines CaEs-17cell growth, and have the timeand dose-dependent maner. After the treatment for72h, the induction of apoptosis wasshown by flow cytometry analysis, which demonstrated that extracts I and II（800μg/mL,100μg/mL, and12.5μg/mL） could induce apoptotic in CaEs-17andQGY-7701cells. The apoptosis rates of extracts I on CaEs-17and QGY-7701cellswere72.24%,53.61%,45.69%and67.75%,22.19%,18.41%, which were60.59%,48.27%,34.67%and43.54%,20.99%,14.68%of extracts II. The cell morphologicalchange was observed through inverted microscope. The adherent ability decreased,debris increase and obviously deteriorated state of QGY-7701cells were observed.The protein expression levels in QGY-7701cells were detected by Western blot aftertreated with extracts I and II for48h. The expression level of Bcl-2wasdown-regulated in a dose-dependent manner, whereas that in Bax was relativelyconstant. The increase of Bax/Bcl-2ratio may be associated with their antitumor mechanism.At last, the effects in life span on H₂₂, EAC ascites tumor and in tumor growth onS₁₈₀, H₂₂solid tumor were investigated using mice transplanted model in vivo. Theresult suggested that the treatment of extract I could extend the survival time of H₂₂,EAC ascites tumor mice, and inhibit tumor growth of S₁₈₀, H₂₂solid tumor mice.