The Role Of Reactive Oxygen Species And Acid Sphingomyelinasc In Reeeptor Clustering Induced By0.4mT Magnetic Fields

Objective To clarify the mechanism of EGFR clustering induced by power frequency magnetic field. Methods Human amniotic (FL) cells were exposed to a50-Hz sinusoidal magnetic field, the effects of MF on A-SMase activity and ROS level were measured by sphingomyelinase activity assay kit and active oxygen detection technology respectively, and the relationship among50-Hz MF-induced EGFR clustering, A-SMase and ROS was explored by immunofluorescence technique in the present experiment. Results The activity of A-SMase increased at MF exposure for15min and30min, and the differences were statistically significant (P<0.05). MF at0.4mT could enhance the ROS level in FL cells. The difference was statistically significant (P<0.05). However, the increase of ROS level could be completely eliminated by pretreatment with PDTC, a specific ROS scavenger. MF could significantly induce EGFR clustering(p<0.05).The activity of A-SMase increased by MF could be eliminated by PDTC. The difference was statistically significant (P<0.05). Conclusion ROS mediated EGFR clustering induced by50Hz MF exposure, MF exposure enhanced A-SMase activity via increasing ROS level.