| Objective To investigate the effect of extracorporeal shock wave on the change ofosteoprotegerin in blood plasma and osteoprotegerin expression in glucocorticoid-inducedfemoral head necrosis rabbits.Methods Fifty-four healthy adult male Japanese rabbits, random selection18as controlgroup (group A),other36rabbits were given Methylprednisolone Sodium Succinate andlipopolysaccharide for establishing glucocorticoid-induced necrosis models of femoral head,after medicined then randomly divided into model group (group B), shock wave therapy group(group C), each group have18rabbits. At the same time A group were given correspondingphysiological saline. Group C were start given shock wave therapy after the dose to the rabbitin the second week. Each group were randomly selected6rabbits take ear arterial blood afterthe dose to the rabbit in the4,6,8weeks, at the same time use the air embolism method putrabbits to death and get femoral head. Hematoxylin-eosin staining was used to observe thehistological changes of glucocorticoid-induced femoral head,enzyme-linked immuno sorbentassay was used to measure osteoprotegerin content in blood of rabbits, immunohistochemicalstains observed OPG positive expression in glucocorticoid-induced femoral head and theexpression was semi-quantitative analysised.Results Plasma OPG content: there was no difference between B group and C group at week4after the dose to the rabbit (P>0.05),but both was lower than A group（P<0.05）. At week6,8，both B group and C group was lower than A group（P<0.05）, C group was higher than Bgroup(P<0.05).Positive expression of OPG in femoral head: At week4,6and8after the dose tothe rabbit, both B group and C group was lower expression than A grou（pP<0.05）, but C groupwas higher expression than B group(P<0.05).Conclusion ESW can promote OPG expression in femoral head of glucocorticoid-inducedfemoral head necrosis rabbits and raised the OPG content in rabbit blood plasma then inducenew bone formation. |
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