| Objective：To study the effects of allogenic chondrocytes seeded onto type I/III collagenmembrane on repair of rabbit knee joint articular cartilage defects.Methods：Chondrocytes obtained from knee articular cartilage of rabbits were seeded ontotype I/III collagen membrane and grown in vitro. These allogenic chondrocytes-collagencomposites were then implanted into full thickness central defects created in the patellar groove.A further12rabbits served as a control group. In8,12weeks postoperatively, the specimenswere sacrificed and repair effects were observed via histological staining method.Results： Chondrocytes attached to the surface of the membrane could secrete theextracellular matrix on the membrane. In the transplantation group, the defects were filled withhyaline-like cartilage at the12th week.The cartilage and the subchodral bone remodeled12weeks after transplantation, immunohistochemical staining on newly formed cartilage was moreintense for type-II collagen in the matrix. In the control groups, the defects were filled withfibrous tissue. The semiquantitative histological scores were signifcantly higher in thetransplantation group than in the control group (P<0.01).Conclusions：These fndings suggest that allogenic chondrocytes seeded onto type I/IIIcollagen membrane can effectively enhance the repair effect of full-thickness cartilage defects inrabbits. |
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