The Effect Of Apoptosis And Expression Of HDAC4and P21in Endometrial Cancer Cell By Apicidin

Objective: To investigate the effect of HDAC inhibitor apicidin on apoptosis andexpression of HDAC4and P21in endometrial cancer cell.Methods:Three groups of cells were cultured for this study including normal endometrial epithelialcell (EEC), well differentiated endometrial cancer cell lines HEC-1B and poorly differentiatedendometrial cancer cell lines KLE. HEC-1-B and KLE cell were treated by HDACI apicidin at0,0.5,1.0μ mol/l for24h and48h respectively. The rate of apoptosis was examined by flowcytometry technique. The expression of HDAC4and P21were examined in EEC, HEC-1-B celland KLE cell by western blot. The expression of HDAC4and P21were examined in the twocancer cells treated by0.5μ mol/l apicidin for24h.Results:1.①The apoptosis rate of KLE cell treated by0,0.5,1.0μ mol/l apicidin after24h were(7.45±1.88)%,(17.47±2.08)%and (40.84±1.75)%, while the apoptosis rate of HEC-1-B cellwere (4.01±0.46)%,(11.28±1.32)%and (16.76±1.35)%. The rate of apoptosis of HEC-1-B celland KLE cell was enhanced with the prolonged of the time by apicidin (P<0.05).②Theapoptosis rate of KLE cell treated by0,0.5,1.0μ mol/l apicidin after48h were (12.02±0.37)%,(21.53±2.18)%and (57.10±1.43)%, while the apoptosis rate of HEC-1-B cell were (6.59±0.75)%,(14.07±1.65)%and (23.80±1.70)%. The apoptosis rate of HEC-1-B cell and KLE cell wereenhanced with the prolong of the time by apicidin (P<0.05).③The result of statistical analysisshowed that the apoptosis rate of HEC-1-B cell and KLE cell were enhanced in a time andconcentration dependence manner by apicidin (P<0.05), and the apoptosis rate of KLE cell washigher than the apoptosis rate of HEC-1-B cell by apicidin in the same time and concentration(P<0.05).2.①The expression of HDAC4in normal endometrial cell, HEC-1-B cell and KLE cellwere (0.69±0.04),(0.82±0.02) and (0.94±0.04). The expression of P21in normal endometrialcell, HEC-1-B cell and KLE cell were (1.09±0.03),(0.85±0.09) and (0.69±0.09). The result ofstatistical analysis showed that the expression of HDAC4in normal endometrial cell, HEC-1-Bcell and KLE cell were increased, and P21was decreased (P<0.05).②The expression ofHDAC4in HEC-1-B cell and KLE cell treaded by0.5μ mol/l apicidin were (0.73±0.04),(0.76±0.03). The expression of P21were (1.04±0.05),(0.90±0.09). The result of statistical analysis showed that the expression of HDAC4was reduced and P21was increased afterHEC-1-B cell and KLE cell treated by apicidin(P<0.05).Conclusions:1. Apicidin can promote the apoptosis of the endometrial cancer cells in a time andconcentration dependence manner.2. The content of HDAC4and P21may be associated with the differentiation degree ofendometrial cancer cell.3. Inhibition of HDAC4to up-regulation of P21may be one of the reasons that induced theapoptosis of the endometrial cancer cell by apicidin.