Metformin Facilitate Differentiation Of Rat Bone Marrow Stromal Stem Cells Into Osteoblasts

Objective:To study the influence of metformin (MF) on differentiation of rat bone marrow stromal cells in vitro.Methods:To culture bone marrow stromal stem cells of3week-old male SD rat, after many cell generations, treated with different concentrations of MF respectively, then assay the viability of bone marrow stromal cells and the activity of alkaline phosphatase (ALP). And detect the capacity of osteoblast cells calcification mineralization using Alizarin Red-S staining.Results:To observed MTT, ALP activity and mineralization of osteoblasts cell by calcification detection, the cell number of metformin group are all higher than the control group,400umol/L group is particularly notable; The ALP activity of this group is significantly higher than the control group and the other experimental groups, the number of mineralized nodules of this group is also significantly increased than the control group and the other experimental groups.Conclusion:Metformin dose-dependently facilitate differentiation of rat bone marrow stromal cells into osteoblasts, which promote bone formation, in the concentration of400μmol/L could reach maximal effect.