Primary Cultured Bone Marrow Mesenchymal Stem Cells Treat Traumatic Brain Injury On Rat’s Model

Background:Traumatic brain injury is a common disease of the central nervous system andfrequently-occurring disease. It can be directly destroy intracranial structures, causing thenervous system function in patients with serious, permanent damage, even can lead to plantssurvival state or endanger lives.Bone marrow mesenchymal stem cells is the one which have great potential ofdifferentiation. Domestic and overseas scholars has launched research for using these stem cellsto repair the irreversible damage of nervous system.Objective:In this study, we cultured rat’s the primary cultured bone marrow mesenchymal stem cells invitro, and created rat’s model of traumatic brain injury. Then, we explored the method of curingthe traumatic brain injury by transplanting rat’s stem cells.Methods:A total of 20 adult male SD rats, whose weight fluctuations in 300±20g, were randomlyassigned to of the treatment group and the control group. It was followed modified Feeney’smethod to built models. We selected the location by rat’s stereotactic navigation instrument, with300 g·cm capability to shock rat’s cortex. 24hours later, wo did modified neurological severityscores for them. An adult male SD rat, which is 2 months old, was chosen to be the source ofbone marrow mesenchymal stem cells. Extracted from bones, bone marrow mesenchymal stemcells were cultured in hatch box. The treatment group was transplanted by bone marrowmesenchymal stem cells, and the control group was injected by normal saline. 14 days later, wedid modified neurological severity scores for them again. The rats were put to death for braintissue, which was used to make HE dyeing and microvessel density.Results:1．Histopathology: In the treatment group it was discovered that the thanatosis area wassmaller than the control group;2．Modified neurological severity scores: The score of the treatment group was lower thanthose in the control group（P <0.05）;3．Microvessel density:It showed different statistically between the treatment group and the control group. The microvessel density of the treatment group was lower than those in thecontrol group（P <0.05）.Conclusions:1．The method of curing the traumatic brain injury by transplanting bone marrowmesenchymal stem cells is significantly curative on neurological function recovery;2．The microvessel density of treatment group, which is transplanted by bone marrowmesenchymal stem cells, is hihger;3．The bone marrow mesenchymal stem cells can help traumatic brain injury model restoreneural function, and promote blood essel growth. Speculately, it repair brain tissue by promotingblood essel growth;4．Transplanting the primary cultured bone marrow mesenchymal stem cells for earlytreatment of acute traumatic brain injury is significatly.