The Expression And Clinical Significance Of CD146and Ki67in Leiomyoma Of Uterus And Cellular Leiomyoma

Objective To determine the Cell adhesion molecule CD146, and the proliferation cell nuclear antigen Ki67in the uterine leiomyoma, the cellular Leiomyoma, explore their role in the development of uterine Leiomyoma and its relationship with clinical pathological indicators, and may further provide the basis for clinical differential diagnosis and prognosis.Methods Select30cases of Leiomyoma (uterine leiomyoma, UL), and15cases of cellular Leiomyoma(cellular leiomyoma, CL), the immunohistochemical PV-9000method (immunohistochemistry, IHC) Was used to detect the expression of CD146and Ki67in uterine leiomyoma tissue, cellular uterine leiomyoma tissue and in30cases of the corresponding normal myometrium tissues beside hysteromyoma, And analysis of its expression.Results1. the Cell adhesion molecule CD146positive reaction is located in membrane and (or) cell pulp Shang, for brown yellow particles; The positive expression rates in the tissues beside hysteromyoma (86.67%), the uterine leiomyoma(90%), the cellular uterine Leiomyoma tissue(93.33%),its positive rate trends is increasing, there is statistically significant differences in three groups expression(X2=17.10, P=0.009). The expression of CD146in the cellular uterine Leiomyoma tissue was higher than the tissues beside hysteromyoma (X2=15.461, P=0.001);Comparison of UL group no significant difference(X2=0.506,P=0.918); Comparison the uterine leiomyoma and the tissues beside hysteromyoma of significant difference (X2=13.510,P=0.004).2. Ki67positive reaction is located on the nuclear, for light yellow or brown yellow particles; The positive expression rates in the tissues beside hysteromyoma (0), the uterine leiomyoma(6.7%), the cellular uterine Leiomyoma tissue(30.0%),its positive rate trends is increasing, there is statistically significant differences in three groups expression (X2=13.500,P=0.001). The expression of Ki-67in the cellular uterine Leiomyoma tissue was significantly higer than the uterine leiomyoma and the tissues beside hysteromyoma (X2=5.414,11.250;P=0.02和0.001). Comparison of UL group and the tissues beside hysteromyoma no significant difference (X2=2.069,P=0.150).3. The expression rates of CD146、Ki67in uterine leiomyoma tissue and cellular leiomyoma tissue were not related to theirquantities ages of patients, tumor sizes, the number of tumor (P>0.05).4.Correlation analysis showed that:The expression of CD146and Ki67was positive correlation, cellular Leiomyoma group(rs=0.779, P=0.004). The expression of CD146and Ki67was no correlation,uterine Leiomyoma group(rs=0.340, P=0.067).Conclusion1. The positive expression rate trend of CD146is increasing in the tissues beside hysteromyoma、the uterine leiomyoma tissue and the cellular uterine Leiomyoma tissue. The cell adhesion molecule CD146expression in tumor tissue tested positive in significantly higher than the tissues beside hysteromyoma, prompting CD146exception may plays a role in the occur and development of the UL and CL2. Ki67in uterine Leiomyoma and uterine muscle tissue has a lower rate of expression, showed thatthe uterine Leiomyoma cell with low proliferative activity, as a benign tumor.Biological behavior of cellular Leiomyoma Was different from the uterine Leiomyoma, with malignant behavior tendencies.expression of Ki67incremented as important indicator of prognosis of UL and CL3. Positive expression of CD146、Ki67of Leiomyoma and cellular Leiomyoma no correlation between clinical pathology parameters.4. The positive correlation between CD146and Ki67in cellular uterine Leiomyoma tissue suggest that there is a link between CD146cell adhesion molecule expression and tumor cell proliferation activity increases.combined CD146and Kil67can make to develop a useful point of view on UL and CL should arouse our high attention to and recommendations for strengthening after follow-up.