The Expression And Effect Of Autophagy In Lung After Hip Fracture In Elderly COPD Rats

Objective:1、to establish the COPD model in elderly rats、the elderly rat model of hip fracture.2、observed in elderly COPD rats、rats elderly COPD hip fracture autophagy exists comparing different time points of autophagy expression; Observed in elderly COPD rats elderly COPD hip fracture level of inflammation in rats.3、macrophage inhibitor3-MA elderly COPD hip fracture of the expression of rat bite-associated protein LC3B, as well as the evaluation of the impact of3-MA on the elderly COPD hip fracture in rats of pneumonia.Method:1.15only aged male Wistar rats were randomly divided into three groups:elderly COPD control group of five aged rats in the control group of five hip fractures of aged rats5.Packet after the fumigation method produced a model of COPD and homemade blow to the production of hip fracture model.Observed in elderly COPD control group of aged rats in the control group of lung biology and histological changes observed in aged rats hip fracture group hip image changes.2.30-aged male Wistar rats were randomly divided into2groups, elderly COPD rats, rats elderly COPD hip fracture, respectively8h,12h,24h,3D,5D, rats were killed, ELLASA observed lung inflammatory mediators (TNFa, IL6, of IL10) expression; using RT-PCR, mRNA expression of macrophage-related gene LC3B, according to the PCR results expression in LC3B, the peak of transmission electron microscopy observation of autophagy, using immunohistochemical staining detects self-expression of macrophage-related protein LC3B.3.55aged male Wistar rats were randomly divided into three groups, the elderly COPD rats, rats elderly COPD hip fracture in elderly the COPD hip fracture rat+3MA in the intervention group, the latter two groups are divided into injury8hl2h,24h,3D,5D, rats were killed, each set of lungs, peripheral blood inflammatory mediators (TNFa, IL6, of IL10) expression with ELLASA observed; macrophage-related gene LC3B mRNA expression using RT-PCR, PCR resultsexpression in LC3B peak of the TEM cell autophagy, immunohistochemical method to detect autophagy related protein LC3B, expression.Results:1. And aged rats in the control group compared to older COPD rats group lung morphological changes, and imaging of hip fracture group of aged rats modeling success rate of90%and anatomical results of the assessment results with the x-ray assessment.2. RT-PCR assay showed that the COPD group than the control group significantly increased, and as time continues to rise, reached a peak at24h after injury, has been to the5D after injury, expression of self-bite related genes LC3B mRNA expression; TEM results, Autophagy in the cells of the peak of the COPD group than the control group more, the difference was statistically significant (P<0.05); immunohistochemical results are consistent with the TEM results.3. ELLS A results,3MA can significantly reduce levels of inflammation in the rat hip fractures in elderly COPD, we observed five time points on3-MA group than in the COPD group LC3B, mRNA expression was significantly lower difference statisticssignificance (P<0.05).TEM results and the results of immunohistochemistry and mRNA.Conclusion:1.Successfully established rat COPD model and hip fracture.2. Elderly COPD rats after hip fracture can detect the expression of lung tissue of autophagy、Significantly higher levels of inflammation than the control group.3.3-MA can inhibit the expression of autophagy in the lung tissue, can reduce the level of lung tissue inflammation in the elderly COPD rat hip fracture.