Effect Of Salvianolic Acid B Postconditioning On Morphology And Biomechanics Of Decellularized Porcine Aortic Valve

Objective:To observe the effect of salvianolic acid B postconditioning on morphology and biomechanics of decellularized porcine aortic valve, providing theoretical support for tissue engineering.Methods:Fresh aortic valves from porcine of6to7month were divided randomly into three groups(40valve leaflets in each group).Porcine aortic valve leaflets were treated by two different methods. Control group1involved treatment with Triton-x100、sodium deoxycholate、DNase and RNase.and group2were treated by Triton-x100、sodium deoxycholate、DNase and RNase, then using salvianolic acid B post-processing. Eath samples were observed by microscope and Scanning electron microscope, and the levels of DNA content to confirm the removal of cell. Biomechanics characteristics measured by biomechanics properties-maximum deflection, elongation rate and max tensile stress.Statistical analyses were performed to compare the thickness.Results:No significant difference in tissue thickness was found between two methods (P>0.05),Tx-sal method can protect the heart valve three-dimensional structure. Biomechanics properties-maximum deflection, max load and elongation rate were increased significantly in the group with salvianolic acid B.Conclusion:Salvianolic acid B post-processing can effectively protect the Collagen and elastic fibers and improve the biomechanical properties of decellularized porcine aortic valve.