| Background:Osteoarthritis is a common clinical joint degenerative disease, and the majority people with the disease is in older adults. It mainly involves the knee joint and weight joint, induses illness parts painful and swelling, gradually leads to the loss of joint function, seriously influences patients daily activities, or disability. And the medical expenses are huge. Because of no obvious radiographic change in the early OA, traditional method that rely on imaging change and clinical symptoms to diagnose of OA cannot promptly early diagnosis, lead to illness delay. So how to make early diagnosis of OA is still one of the difficult problem. With the development of molecular biology, the function of biological markers (biomarker, BM) in body fluids in the diagnosis of OA draw attention of scholars day by day. We hope make early diagnosis of osteoarthritis before appear in imaging change through the study and clinical application of BM, and forecast the possibility of organization structure damage occurred and the progress of the disease, and evaluate the effects of the treatment. The existing research are still not completely affirm BM in early diagnosis of OA, and most of the research have their limitations:the first is using imaging standards for the patient degree assess, however imaging change doesn’t accurately reflects the degree of cartilage lesions, there are certain error; In addition the samples used for experiment are the blood or urine of patients, the two kind of samples reflect the systemic metabolic situation more, and not an accurate reflection of local articular cartilage lesions degrees, these are likely to affect the results of the experiment.Objective:Discusses the correlation between CTX-Ⅱ concentration n P II CP concentration as well as the the ratio of the two BM in OA knee joint and the degree of damage, and for the disparity between the sexes.Methods:Collect47patients (48total knee)who do arthroscopy or joint replacement and extraction of joint fluid in our hospital from2009to2011, male25cases (25knee), female22patients (23knee)。 In cases of arthroscopic surgery or total knee joint replacement, we take the lateral approaches do the knee joint stock liquid extraction with0.5～5ml, then centrifugal in the4500rpm,20minutes conditions within two hours, and uses Eppendorf tube to enrol supernatant, quick frozen, kept in-70℃and to be measured. During the knee arthroscopic surgery or total knee joint replacement, use Outerbridge method to evaluate the cartilage damage. And divides the samples into five groups (0points,1points,2points,3points,4points)according to the highest rating score, divides the sample into two groups (<10points group and≥10points group) according to the cumulative score, divides the samples into two groups of men and women according to gender. To determine CTX-Ⅱ (USCN, E90686Hu) and PIICP(USCN,90964Hu) in joint fluid by ELISA method.Results:Either the CTX-Ⅱ and PIICP concentration in joint liquid as a single index or the ratio, the differences are not statistically significant in different degree of damage of articular cartilage (P>0.05), and no correlation with cartilage damage (P>0.05). But CTX-Ⅱ concentration and PIICP concentration has positive correlation in the fluid of joint (P<0.05). In addition, the differences of the CTX-Ⅱ concentration between men and women is not statistically significant (P>0.05), and the differences of PIICP concentration and of CTX-Ⅱ/PIICP value between men and women is statistically significance (P<0.05).Conclusion:In this study, the CTX-Ⅱ and PIICP concentrations as a independent index have no confirmed role in the prediction of the occurrence and development of OA. And it shows no statistically significant results while combining both BM, but it may perhaps have a new found while make dynamic monitoring of BM. So the future research should combine a variety of BM, and make dynamic monitoring of the concentration, and should consider age and gender factors at the same time. |
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