Expression And Clinical Significance Of Raf Kinase Inhibitor Protein And Phosphorylated Raf Kinase Inhibitor Protein In Gastric Carcinoma

Background and ObjectiveGastric cancer incidence and mortality rates in the forefront of our malignant tumors, in most cases the time of diagnosis has been the case in late. The current use of combined therapy with surgery, but many patients eventually die of local recurrence and distant metastasis, the5-year survival is only30%to40%, the treatment effect is still not ideal. Studies have shown that gastric cancer of poor prognosis in gastric cancer cells to proliferate indefinitely, and the reason may have changed many of the genes associated with cell proliferation, to identify these tumor cell proliferation-related genes and clarify their cancer and Role in the Development of Cancer Prevention and Treatment of great significance. The incidence of gastric cancer in the world, there is a clear downward trend, but the decline in death rates is not obvious, gastric cancer still accounts for a variety of Cancer Mortality in the first two. Gastric cancer in2/3of cases in developing countries, especially Japan, China and other East Asian countries of high incidence.In China each year of new gastric cancer cases by about400000deaths of300,000, is not only a serious threat to human health, while also exposes patients to society as a whole to withstand the enormous economic burden, is an important factor in rapidly rising medical costs. Conservative calculations, if each gastric cancer patients with medical expenses of$10,000, the additional costs of$4billion a year. Gastric cancer patients found that most are in late clinical cure rate is very low, and spent a lot of manpower and material resources. In addition, patients with gastric cancer more than men in the prime of life, social and family labor, a gastric cancer patients with an enormous burden to society, families and individuals, and pain. Gastric cancer is a gradual occurrence of the disease, usually in the early asymptomatic, and the vast majority of patients found to have entered the middle and late. Patients of our country are mostly due to disease treatment, early diagnosis of gastric cancer rate is very low, less than10%of early gastric cancer after surgery5-year survival rate was90%, II and66%, Ⅲ51%, while the advanced gastric cancer only14%. For gastric cancer prevention is better than cure, through the adjustment of public health resources and strategies, the strategy forward, the focus from treatment to the cancer incidence and improve the cure rate, has become the consensus of the global cancer researchers.The current status of gastric cancer prevention and control in China is a third year, the incidence of high mortality, early diagnosis is low, low rate of radical resection, the5-year survival rate. Advanced international level of gastric cancer prevention in Japan, South KoreaCompared with China in the prevention and treatment of gastric cancer is still a considerable gap, may be related to the diagnosis, treatment, and even economic, social and other various factors, one important factor is the stage of disease when treatment is late. Therefore, actively carry out effective screening to improve early diagnosis to improve the survival rate of gastric cancer, an important means of reducing mortality.To investigate the expression of Raf kinase inhibitor protein (RKIP) and Phosphorylated raf kinase inhibitor protein (P-RKIP) in gastric carcinoma and their relation with the invasion and metastasis of gastric carcinoma.Materials and methodsSelect the hospital from January2002to January2010Gastric Cancer Surgical Specimens of80cases. The selected conditions were:did not receive preoperative chemotherapy and radiotherapy and immunotherapy for the first time to accept the surgical treatment of patients with gastric cancer;49cases were male patients, female patients of31cases. All surgical specimens were confirmed by pathology, according to histological type and degree of differentiation is divided into two groups:high medium differentiation group of65cases and25cases of poorly differentiated group, the former including papillary and tubular adenocarcinoma, which contain a poorly differentiated adenocarcinoma, mucinous adenocarcinoma, signet ring cell carcinoma and undifferentiated carcinoma. Immunohistochemistry was used to detect the expression of RKIP and P-RKIP in gastric carcinoma, paratumor tissues. Statistical analysis was used to determine the relationship between their expression level and clinicopathological parameters.ResultsStatistical analysis revealed that the expression level of RKIP and P-RKIP in tumor tissue were significantly lower than that in paratumor tissues (P<0.05). RKIP and P-RKIP expression are dramatically lower in gastric carcinoma with lymphatic metastasis than that without respectively. In moderately and well differentiated gastric carcinoma both RKIP and P-RKIP positive stain rate is significantly higher than that in poorly differentiated ones. There was a positive correlation between RKIP and P-RKIP expression in gastric carcinoma and in paratumor tissues.ConclusionThe expression of RKIP and P-RKIP were down-regulated in gastric carcinoma tissues. And the expression level of RKIP and P-RKIP were correlated with the carcinoma’s differentiation and lymphatic metastasis.