| Objective:To evaluate the effects of Sarcandra glabra particle on peripheral blood system and ROS in miniature pigs irradiated by y-rays delivered to the parotid gland, to study the possible protective mechanism and the radioactive damage mechanism of parotid gland.Methods:(1)Model parotid radioactive damage:Forty-five male miniature pigs were randomly divided into control group, radiation group and radiation plus medication group, and each group contained3parallel groups (subgroup a, b and c). From1week before exposure of the parotid gland region to DT15Gy y-ray irradiation(which was not administered in the control group), the miniature pigs in radiation plus medication group were given Sarcandra glabra powder with0.3g/Kg, while those in other groups received an equal amount of saline.Bilatereal parotid glands were taken and weighed on the days10,40,and90following the exposure in subgroups a,b,and c,respectivelly.(2) Blood was drawn from vena cava for routine examination and serum chemistry test in1day before and10days after radiation respectively.(3) ROS content in the parotid glands were determined by ROS enzyme-linked immunosorbent assay kit. Results:(1) Established the model of paotid gland radioactive damage intervened by Sarcandra glabra successfuiiy.(2) The WBC and RBC count.vas significantlly reduced after irradiation,but the count of the WBC and the RBC were significantlyhigher in radiation plus medication group than in the radiation group (P<0.01); the count of the PLT of the radiation group was significantlly recdued,but it has a higer count in radiation plus medication group,and the difference was statistically significant (P<0.01);the content of LDH was significantlly reduced after irradiation(P<0.01), but the content of the LDH was significantly higher in radiation group than in the radiation plus medication group (P<0.01); the content of AMS was significantlly increased after irradiation(P<0.01), but the content of theAMS was significantly higher in radiation group than in the radiation plus medication group (P<0.01);(3) The content of ROS was significantly lower in radiation plus medication group than in the radiation group (P<0.01). In the radiation plus medication group,the ROS content showed no significant difference between subgroups a and b or between subgroups a and c(P>0.01),but differed significantly between subgroups b and c(P<0.01). Sarcandra glabra showed a strong ROS-scavenging effect10days after the irradiation,and the ROS content was similar with that in the control group(P>0.01); at40and90days, the ROS-scavenging effect of Sarcandra glabra was still observable,but the ROS content was significantly higher in the irradiation plus medication group than in the control group(P<0.01).Conclusions:(1) It can model parotid gland radioactive damage successfully with illuminating parotid gland issues with the γ ray DT15Gy single dose;(2) Pretreament with Sarcandra glabra improved the irradiated miniature pig’s peripheral blood system. Sarcandra glabra can promote the recovery of the number of the WBC and the RBC,slowing down the AMS increase, increase the number of the PLT and the LDH; Sarcandra glabra probably by facilitating the peripheral blood recovery so as to improve the immunity of the organism,which may be the reason for the radioprotective effect of Sarcandra glabraagainst γ-radiation-induced acute injury to parotid gland of the minipig.(3) Sarcandra glabra displays a ROS-scavenging effect in the parotid gland of miniature pigs against irradiation, especially at10days following the exposure,which may serve as the main mechanism for the protective effect of Sarcandra glabra against radiation injury in the parotid gland. |
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