The Expression And Change Of IGF-1and Annexin A1in Medial Rectus Of Experimental Strabismus Kittens

Objective To investigate the expression of IGF-1and Annexin A1of medial rectus in optically induced concomitant extropia in kittens. To explore the role of IGF-1and Annexin1in the occurrence and development of concomitant extropia.Methods fifteen kittens were devided into two groups random:experimental group and control group.Nine xperimental kittens were fitted with optical prims glasses. Based on the durition of wearing glasses,experimental group were divided to A、B and C three groups.Taken the Experimental and control kittens’s medial rectus after the first、second and third months respectively. Observation medial rectus morphological performance after HE dyed.Immunochemical method measured the mean optical density according to the expression of IGF-1and Annexin1in medial rectus.To statistics the average light density values among groups.Results①The expression of IGF-1were lower in experimental group than control group, mean optical density are0.300±0.012、0.265±0.038and0.195±0.028.respectively. Compared with controls,they were significant differences (P<0.05).②The expression of AnnexinAl were decreased in experimental group.Compared with controls,the difference waas statistically significant (P<0.01).③The expression of IGF-1and AnnexinAl are decreased gradually in experimental groups,they were significant differences (P<0.05).④The expression of IGF-1and AnnexinAl have no notable variation,there is no statistically significant(P>0.05)Conclusion The lower expression of IGF-1and Annexin1in strabismus cats may participate in the incidence of concomitant extropia; With the duration of wearing optical prims glasses prolong, the level of IGF-land Annexin A1is decrese gradually, we consider it may be the cause of generation and development of concomitant extropia. And wearing optical prims glasses can be useed as a experimentsl method to make strabismus animal model.