Expression Of Adiponectin And Its Receptors In Patients With Primary Gouty Arthritis

Objective: To study relevant molecular expression of adiponection(ADP) and its receptors in peripheral blood from patients with primary goutyarthritis (GA) and healthy individuals; to explore the role of ADP in thepathogenesis of GA.Methods: (1)88 GA patients and 80 healthy controls (NC) were enrolledinto the study. The clinical data of primary gouty arthritis patients and healthyindividuals were collected. PBMC were isolated from blood in vitro. (2)Thelevels of ADP in plasma of primary gouty arthritis patients and healthyindividuals using enzyme linked immunosorbent assay (ELISA). (3)Real timequantitative polymerase chain reaction (RT-qPCR) was employed to study theexpression of ADR1 mRNA and ADR2 mRNA in PBMC from GA and NC,and the differences of genes expression in two groups were compared,Spearman correlation and multiple regression analysis were used to evaluatedrelationship between mRNA level and the index which were detected.Results: (1) The concentration of plasma ADP was significantly lower inGA than NC (6.32±6.72μg/ml vs 8.36±6.37μg/ml, P<0.05). (2)The expressionof ADR1 and ADR2 mRNA in GA was significantly increased compared tothe NC (ADR1: 0.0858±0.0784 vs 0.0458±0.0337, ADR2: 0.0122±0.0164 vs0.0054±0.0024; P <0.05, respectively). (3)In GA patients, the level of ADPwas negatively correlated with LY and UA(r=-0.36, r=-0.32, P<0.05,respectively), positively correlated with SR and LDL(r=0.31, r=0.39, P <0.05,respectively); the expression of ADR1mRNA was negatively correlated with TG (r=-0.43, P<0.05), positively correlated with SR, CRP(r=0.45, r=0.57,P<0.05, respectively); the expression of ADR1mRNA was negativelycorrelated with GLU and UA(r=-0.50，r=-0.59，P<0.05, respectively). (4)Multiple regression analysis show that age was the independent factor forADP, UA was the independent factor for ADR1mRNA, and TG, UA, LDL,GR were the independent factors for ADR2mRNA in GA patients.Conclusion: Altered expression of ADP and its receptors mightparticipate in the pathogenesis of primary gouty arthritis.