Expression Of MicroRNA In The Peripheral Blood Of Pancreatic Cancer And Its Clinicopathological Significance

Objective:To detect the expression of plasma microRNA in pancreatic cancer patients and the nomal, and evaluate its clinical significance as biochemical marker in diagnosis, then provide a new way to diagnose pancreatic cancerMethods:37cases of pancreatic cancer patients were included in this study, and10cases of healthy volunteers were used as control. Reverse-transcription polymerase chain reaction (RT-PCR) was used to detect the expression of microRNAs, U6was used as internal control. The relationships between clinicopathoiogic characteristics of pancreatic cancer and the microRNA expression were analyzed.Results:1. The relative abundance of plasma microRNAs were significantly higher than in the control group. The relative abundance of plasma microRNAs were miR-190:3.50±4.07; miR-196a:4.21±4.13; miR-221:4.00±4.11; miR-222:2.57±2.55.2. The highly expressed plasma miR-190, miR-196a, miR-221, miR-222levels did not correlate with clinicopathologic characteristics such as sex, age, tumor maximal diameter and the level of serum CA199(P>0.05).3. Plasma miR-196a levels showed positive correlation with TNM stage in PDAC patients.Conclusion:1. plasma miR-190, miR-196a, miR-221and miR-222were highly upregulated in pancreatic cancer patients.2. Plasma miR-196a levels showed positive correlation with TNM stage in PDAC patients.3. To detect the expression of plasma microRNAs may provide us a new way to diagnose pancreatic cancer in an earlier stage.