The Expression And Relationship Between SOX2and Oct4in Esophageal Squamous Cell Carcinoma

[Background and Objective]The incidence and mortality of gastrointestinal tumors has not been an exception andincreased year by year, especially the incidence accounted for2%of all malignant tumorsappears in the esophageal epithelium of digestive tract cancer, every year which can takeaway about22million lives. So that esophageal cancer is the digestive tract cancer aftergastric cancer and liver cancer mortality cancer. The exact etiology and pathogenesis ofesophageal cancer remains unclear.The cancer stem cell theory (cancer stem cell hypothesis) believes that the tumorderived from the stem cells with spontaneous mutations or induced mutations, is amalignant proliferation of the culprit and one of the main reason for tumor recurrence.Usually we called stem cells that have a self-replicating and self-renewal capacitytotipotent cells. Stem cells in the body when provide the appropriate conditions, candifferentiate into any required in the body during the development of a functional cell.Recently, the roles of SOX2and Oct4have been studied in human cancers, Forexample in gastric cancer, Colon cancer and so on, and there is a certain relationship withtumor metastasis and prognosis. To further understand the role of SOX2and Oct4inesophageal cancer development, We design using immunohistochemical SP assay SOX2and Oct4in esophageal cancer and its matching expression of the adjacent tissues, andanalyze them with the relationship between the clinical and pathological data, and theirexpression in esophageal cancer correlation; Western blotting (by Western-blot) detectionof esophageal squamous cell carcinoma cell line EC109and EC9706in SOX2protein and Oct4protein expression and to clarify their role in the development of cancer occurrence.[Methods]The complete collection of pathological and clinical data in January2011to October2011, the Xi jing digestion Hospital, surgical resection and Pathology examinationconfirmed esophageal squamous cell carcinoma and its matching tissue beside the cancer43cases, including29cases of male14cases, female, age38to80years, mean65years,which well-differentiated with the differentiation of15cases,28cases of poorlydifferentiated, with lymph node metastasis in25cases, no metastasis in18cases. ByImmunohistochemiscal Streptavidin Perosidase method to detect SOX2and Oct4inesophageal cancer and its matching expression of adjacent tissues, By Western blotdetection of esophageal squamous cell carcinoma cell line EC109and EC9706in SOX2protein and Oct4protein expression.[Results]1. SOX2expression in esophageal squamous cell carcinoma positive percentage of60%(26/43cases) was significantly higher than their matched cancer adjacent tissues19%(8/43cases), suggesting that SOX2expression in esophageal squamous cellcarcinoma increased (P<0.05).2. Positive percentage of Oct4expression in esophageal squamous cell carcinomawas81%(35/43cases), significantly higher than their matched cancer adjacent tissues16%(7/43cases), suggesting that the Oct4expression in esophageal squamous cellcarcinoma increased (P<0.05).3. The expression of SOX2and Oct4were significantly associated with tumordifferentiation and lymph nodes metastasis in ESCC(P<0.05).4. SOX2and Oct4expression showed a negative association with age, gender andTNM stage of patients in esophageal squamous cell carcinoma（P>0.05).5. The relationships between SOX2and Oct4expression in esophageal cancertissues were analyzed statistically(P<0.05).6. SOX2and Oct4were co-expression in EC109and EC9706. [Conclusion]SOX2and Oct4in esophageal squamous cell carcinoma and cell lines showed highexpression, Positive relationship were between in esophageal squamous cell carcinoma ofSOX2and Oct4expression, suggesting that they may be involved in the development ofesophageal carcinogenesis; And they all expressed highly with the lymph node metastasis,suggesting that they may promote tumor metastasis; Both expressed in esophagealsquamous cell carcinoma of the poorly differentiated group is higher than inwell-differentiated group, suggesting that the two may be associated with tumormalignant phenotype; All abnormal expression in esophageal squamous cell carcinomaand cell lines may be related to the malignant transformation of esophageal squamouscell carcinoma.