The Relationship Between The Level Of Serum1,25（OH）₂D₃and Oxidative Stress In Patients With Diabetic Nephropathy

Objective：To explore the relationship between the level of serum1,25（OH）2D₃andoxidative stress in patients with diabetic nephropathy.Methods：175subjects were enrolled， including75patients with diabeticnephropathy（DN group），50patients with diabetes and without nephropathy （DMgroup）and50healthy subjects（NC group）. DN was defined as urinary albumin-to-creatinine ratio （ACR）≥30mg／g.75DN patients were divided, according tourinary excretion rate, into microalbuminuria group （EDN group）,macroalbuminuriagroup（CDN group）.The level of serum MDA、SOD、CAT、GSH were measured byspectrophotometer assay. The level of serum1,25（OH）2D₃were tested by enzymelinked immunosorbent assay.Results:The level of serum1,25（OH）2D₃in DN group were significantly lower thanin DM group （10.13±1.02vs16.20±1.06, P<0.05）. Compared with DM group andDC group, the level of MDA were significantly higher in DN group （P<0.01）.Still,there were significant differences between different groups of DN patients.The levelof serum SOD,CAT,GSH in DN group were significantly lower than NC group andDM group （P<0.01）. The level of SOD,CAT,GSH in CDN group were significantlylower than EDN group （P <0.05）. The level of CAT,GSH in ESRD group weresignificantly lower than CDN group （P<0.05）. The level of serum1,25（OH）2D₃wasnegative correlation with UAE in DN group （r=-0.452,P<0.05）.The level of serum1,25（OH）2D₃were significant positive correlation with the level of SOD,CAT,GSH inDN group（r=0.286,P<0.05; r=0.390, P<0.05; r=0.671,P<0.05）. The level of serum1,25（OH）2D₃was negative correlation with the level of MDA（r=-0.330,P <0.05）.Conclusions:There is lower serum1,25（OH）2D₃level in DN patients in comparisonwith diabetes patients and heathy subjects.The oxidative stress is enhanced in DNgroup in comparison with DM group and NC group.The Level of serum1,25（OH）2D₃may be correlate with oxidative stress in patients with DN.