Investigation On The Interactions Of Nasopharyngeal Carcinoma CNE-2with Nimotuzumab By Raman Spectroscopy

Objectives: To study the interactions of CNE-2treated with different concentrationand time of Nimotuzumab by Raman spectroscopy. Methods: CNE-2cells werecultured. Cells in good growth state were collected and inoculated into four cellculture media. Nimotuzumab solutions with0，40ug/ml，150ug/ml and200ug/ml wereadded into four cell culture media respectively. The volume of Nimotuzumab in everycell culture medium was2ml. All the cell culture media were cultured for24hour incell incubator. CNE-2cells in four cell culture media were detected by Renishawconfocal micro-Raman spectroscopy.20cells in each drug concentration weredetected. PCA-LDA was employed to analyze the Raman spectroscopy exploringoptimal concentration. CNE-2cells were inoculated into another3cell culture media.2ml Nimotuzumab solution with optimal concentration were added in3cell culturemedia respectively. Cells in three media were cultured in cell incubator for24h,48hand72h, respectively. And then cells were detected by confocal micro-Ramanspectroscopy.Results: After24h in Nimotuzumab injection, Raman spectroscopy of CNE-2cells in40ug/ml wasn’t clearly different from that of control group. There were severalobviously different peaks of Raman spectroscopy between150ug/ml group andcontrol group. Many peaks of200ug/ml manifested obvious difference with controlgroup. After PCA-LDA analysis, the trend of discrimination became more and moreobvious as the increase of drug concentration. Drug group of200ug/ml and controlgroup could be discriminated significantly. The sensitivity for drug groups40ug/ml，150ug/ml and200ug/ml was60%,85%,100%respectively. The specificity for druggroups40ug/ml，150ug/ml and200ug/ml was70%,70%,100%respectively. Ramanspectroscopy of time groups24h,48h and72h with drug concentration200ug/ml manifested obvious difference. However, their spectroscopy didn’t show significanttrend as the increase of drug time.Conclusions: By analysis of Raman spectroscopy, the interaction between CNE-2cells and Nimotuzumab enhanced as the increase of drug concentration when the drugtime was24h. And the interaction of200ug/ml group was most significant. The effectof drug didn’t show significant relationship with drug time, which might be related tothe interference of Raman spectroscopy peak. The improvement of methodologywould help confirm it.