The Experimental Study Of The Effect Of Salidroside On Invasive Ability Of Human Skin Melanoma Cells A375

Objective To investigate the effect of salidroside on invasion ability of human skinmelanoma cell invasion.Methods The human skin melanoma cells A375was cultured in vitro and pretreated withdifferent concentration of salidroside (0.01，0.10，1.00，10.00，100.00μg/mL) ornon-pretreated (control group). The adhesive power of human skin melanoma cells A375on basement membrane was detected after crystal violet staining. The invasion andmigration ability of human skin melanoma cells A375under the influences of drugs wereobserved using transwell chamber model. The adhesive power and invasion and migrationability were compared among the A375cells pretreated with different concentrations ofsalidroside and the control group.Results Compared with the control group, salidroside in each concentration groupsignificantly inhibited the adhesive power of melanoma cells on the basement membrane,and also inhibited their migration and invasion abilities, there were significant differences(P<0.05). With the drugs concentration increased, the inhibitory effects of salidroside onthe adhesive, migration and invasion abilities of melanoma cells were significantlyincreased (P <0.05).Conclusion Salidroside has the effect of inhibiting invasion and migration of human skinmelanoma cells A375.