| Aims: To investigate the expression of PKM2on human gastric cancer cell lines anddefine the biological fuction of PKM2on gastric cancer cells proliferatio, migrationand apoptosis.Preliminary investigate the mechanism of PKM2on gastric cancer celllines, so as to further research and diagnosis of early gastric cancer and moleculartargeted therapy to provide new ideas and theories.Methods: In this study, semi-quantitative RT-PCR and Western-blot technique wasused to validate the PKM2expression on mRNA and protein levels in gastric cancer，and BGC-823cell was applied in this study. The PKM2-siRNA were transfected intothe cells,and the transfected PU6was used as a negative control.Then the silencingeffect was confirmed by Western-blot and qPCR.Furthermore, we preliminary studythe functions and mechanism of the PKM2using CCK8,Transwell technique and flowcytometry analysis methods on gastric cancer cell line BGC-823.Results: At cellular level, PKM2exist expression differences between differentdifferentiation of human gastric cancer cell lines and immortalization human gastricepithelial cells, in which detected from the mRNA and protein levels. With theenhance of cell differentiation, PKM2expression is reduced and the two methods arebasically the same results.The silencing of PKM2gene by siRNA was confirmed, Todetected by the methods of CCK8, Transwell chamber and flow cytometry shows thatcell proliferation, migration and anti-apoptotic capacity reduced than stabletransfected empty plasmid PU6cells after PKM2is disrupted on poorly-differentiatedgastric cancer BGC-823cells.Conclusions: The expression of PKM2is upregulated in human gastric cancer and italso play a important role in promoting growth of gastric cancer. |
PDF Full Text Request