Gene Isolation Related To Resistance To Low Phosphorus In Maize Using CDNA-AFLP Technique

In order to study the molecular and regulation mechanism. In this experiment, separated P-deficiency related gene fragments by cDNA-AFLP technology, and double endonuclease digestion cDNA-AFLP technology was applied to screen isolate and use bioinformatics method to analyze the gene differentially expressed fragments. These gene differentially expressed fragments related to the condition of low phosphorus and the condition of normal phosphorus. The results were as follow.1. Establishing the isolation technology system adaptable for total RNA in roots and leaves, and reverse transcription into double-stranded cDNA. Its integrality was fine.2. Using double endonuclease digestion cDNA-AFLP technology, and120differential expression transcript-derived fragments (TDFs) were obtained, of which78TDFs were cloned.3. Using bioinformatics method to analyze and predict function of sequencing results. Eight gene fragments had neither annotated nor protein function analysis, and others had protein function analysis. So they were divided into10categories, including unknown protein, protein synthesis, cell rescue/defense/virulence, transcription/cellular communication/signal transduction mechnism, energe, metabolism, and protein fate.4. Analyzing nine gene fragments related to P-deficiency into cDNA library,and got their full cDNA sequences. Then used homology analysis, which showed that these6fragments had very high homologies with some other species, such as Arabidopsis and rice. While the remaining three fragments with other species homology were low.