The Extention For Population And QTL Analysis For Agronomic Traits In Chromosome Segment Substitution Lines Of Rice

Most traits of crops are quantitative traits, studying the genetic mechanisms of quantitative traits for crop breeding is very important. As one of the most important food crops, the studying of quantitative traits of rice is very important, and the mapping populations which are used for the studying of quantitative trait loci plays an important role in rice research.On the one hand,using CSSLs derived from Sasanishiki/Habataki cross as original materials, we have expanded three lines (SL402, SL403, SL404) which overlapped the chromosome1of Sasanishiki, and we have analyzed about7agronomic traits of derivative CSSLs including the plant height, flag leaf length and so on, and we have done some related QTL analysis and mapping. On the other hand, the QTL fine mapping of panicle length on chromosome5also be involved in this research based on markers. The main results were as follows: Part1:The extention of SSSLs and the QTL analysis of agronomic traits1. We have selected50derivative CSSLs from separate F2population by making use of the existing molecular markers on chrl. We made substitution lines (SL402, SL403, SL404) cross with Sasanishiki, we got F1, then we got separate F2population. On the whole, we got14,19,17derivative CSSLs from separate F2population of SL402, SL403, SL404separately,and the three derivative CSSLs were named of A、B、C population separately.2.We have finished QTL mapping for A and B population on7agronomic traits by means of Windows QTL IciMapping v2.2.We got43QTLs and important parameters related to these QTLs on7agronomic traits of A and B population. Part2:The fine mapping of qPL5Analyzing the mapping population which is F2and F3generation of SL416/Sasanishiki by making use of interval mapping and markers, we have got the major QTL genes of panicle length qPL5-l and qPL5-2. The QTL mapping intervals of qPL5-1and qPL5-2are SC72-RM2185and SC86-SC98on chr5separately.