Study On Tissue Culture And The Preliminary Study Open-Tissue-Culture Of Lilium Asiatic Hybrids’prato’

Lilium asiatic Hybrids’Prato’is the cultivars of Asiatic hybrids. Orange red flower, with excellent cold resistance, disease resistance ability and open field cultivation in the northeast region, Cultivation of lily varieties are hardy important parents. Taking the bulb scales as explants, the affection of disinfection methods and photoperiod to shoots or calli induction from bulb scales and leaves in vitro was studied; The effective Bacteriostatic agent concentration medium was screened, conducted a preliminary exploration, in order to simplify the process of tissue culture, and reduce the cost of tissue culture on Open tissue culture of Lilium asatic Hybrids’Prato’.1. Taking the bulb scales of Lilium asatic Hybrids’Prato’as explants, the affection of disinfection methods, different growth regulator combination and illumination time on to shoots or calli induction from bulb scales in vitro was studied. The results showed that the best disinfection method was immersing in0.1%HgCl2add Tween20for8min with the highest growth rate. The best induetion medium for Lilium asatic Hybrids’Prato’was MS+0.5mg/L6-BA+0.1mg/LNAA respectively, in which the highest induction rate was85.0%, and the average number of shoots from per explant was3.6; The bulblets of Lilium asatic Hybrids ’Prato’, we seleeted as explants for in vitro culture, and compared their inducing differentiation rate of different position in bulblet, The results of experiments indicated that the containminion rate of inner and middle part in bulblet were in low level, inner and middle scales ability to induce adventitious buds and callus is better than the outer layer, inner scales ability to induce adventitious buds and callus is the best,. The best induetion medium for inner scales was MS+0.5mg/L6-BA+1.0mg/LNAA respectively; The darkness was beneficial for the occurrence of adventitious buds from bulb scales, and MS+1.00mg/L6-BA+0.10mg/L NAA was the best medium.2. The results showed that there is position effect during induction of differentiation in sterilized leaf segments, the adventitious buds differentiation rate; base leaf was the highest, central followed, at the top was almost no differentiation. Light have a certain impact on leaf differentiation, the dark train leaves the differentiation rate is always higher than the optical train at the same Concentration of2,4-D. In dark, the leaves were also easy to generate calli, with the best medium MS+0.10mg/L2,4-D.3. Basal renewable bulblet had a large number of base, value-added quickly, aseedling and no vaccine leaveing. So we celected the receptacle basal renewable bulblet as the best induced explants.The best medium for adventitious buds induced from bulb scales was MS+0.50mg/L6-BA, in which the highest induction rate was100.0%, and the average number of shoots from per explant was4.7. while the best regeneration medium for adventitious buds was MS+0.50mg/LNAA, average to generate a larger number of adventitious buds and roots, and plant growth robust, acquired adventitious buds can be directly used for hardening transplanting.4. During the stage of callus of multiplication and differentiation, it was found That, for Lilium asatic Hybrids’Prato’ bulblet seeding, MS+1.00mg/L6-BA+0.50mg/LNAA was the best medium. in which the highest induction rate was100%, and the average number of shoots from per explant was8.4; for sterilized rooting segments of Lilium asatic Hybrids’Prato’ seeding, the best multiplication medium was MS+0.50mg/L6-BA+1.00mg/LNAA, in which the highest induction rate was76.7%, Adventitious buds quantity and robust growth; Different hormone combinations gave different responses to the effect of buds subeulture multiplication. The treatment with MS+0.50mg/L6-BA+0.05mg/LNAA showed better effect than others; In the medium of1/2MS+0.50mg/LIBA, roots can be the best generated from plantlet, with its highest induction rate86.7%, and the mean number from one plantlet6.0; Through training indoors, the survival rate of the transferred plants was85%, and then grew well.5. Test of Simplify the culture vessel; Contrasting the use of Disinfection of the plastic cups and Sealing membrane type to the influences of medium pollution rate, The results showed that the plastic cups(Potassium permanganate disinfection)+plastic sealing membranes is the optimal, Using potassium permanganate disinfection of plastic cups, medium contamination rate was significantly lower; Mancozeb effective inhibitory concentration is5mg/L in the plastic cups(Potassium permanganate disinfection)+plastic sealing membranes; Open the sterile console ventilation can reduce the rate of pollution, but not with open ventilation group differences. Effective inhibition of potassium permanganate is about16d.6. Open tissue culture regenerative bulblet of Lilium asatic Hybrids’Prato’ differentiation rate was100.0%, and the traditional group training the same, the average differentiation bud slightly lower than the conventional tissue culture for4.4, but less; the open Lilium asatic Hybrids’Prato’medium was screened by researching the survival of buds which no significant difference with traditional Lilium asatic Hybrids’Prato’after inoculationg traditional clustering buds onto the media without high-temperature autoclave. it was found That, Open tissue culture adventitious bud proliferation effect is better than the traditional tissue culture of adventitious buds add value to the induction and proliferation times higher than the traditional tissue culture; IBA treatment60s is the most appropriate lily micro-cuttings, IBA processing time, in which the highest Rooting rate was92.3%. Tissue rooting growth robust of Lilium asatic Hybrids’Prato’, faster than conventional tissue culture to adapt to the environment.