Studies On Somatic Embryogenesis And Genetic Transformation With Blue Gene Of Oriental Lily ’Bernini’

Lily is popular with huge and beautiful flowers, also bright in color. It can be used as cut flower, pot flower and landscaping. Besides, it has medicinal and edible function. The ornamental characteristics of lilium such as florescence, flower color, flower shape, flower fragrance have been studied, flower color is the most important traits, recently, the successful commercialization of transgenic carnations and roses has attracted people’s attention in dream of blue flower color genetic engineering, and it has been extensively studied by more researchers.We have constructed the bianry vector that Phalaenopsis F3’5’H gene with Hyacinth DFR gene or Petunia DifF gene which regulated by CHS specific promoter of Lilium oriental ’Sorbonne’. Here, we establish an efficient regeneration system of Lilium oriental’Bernini’ via somatic embryogenesis, and transform lilium through Agrobacterium-mediated transformation. The results were show as follows:(1) Plant growth regulator of Picloram alone,2,4-D combination with TDZ, are able to induce somatic embryogenesis from scale explant of Lilium oriental’Bernini’, additional PIC allows each explant formed21.9of individual embryos, whereas it’s4.3when additional2,4-D and TDZ, PIC is better for induction of somatic embryos than2,4-D and TDZ in Lilium oriental’Bernini’.(2)The optimal medium for somatic embryogenesis is MS+0.5mg-L-1Picloram+30g·L1sucrose+Vg·L-1agar.(3) The medium for Bulb enlargement is2MS+30g-L-1sucrose+7g·L-1agar.(4) The medium for plant regeneration is MS+30g-L-1sucrose+7g·L-1agar.(5) Hygromycin concentration for selection of somatic embryo is determined to be25mg-L(6) Obtained hygromycin-resistance somatic embryos and bulblets.