| Chilli is a universal and valuable vegetable crop because it is rich in capsaicin, capsanthin,carotene, vitamins and so on. Kasugamycin and carbendazim are used to protect the plantsfrom damage and infestation by bacteria in pepper planting process. In recent years, it is on theincrease that the number of test items of which the chilli for import and export should be tested ofgovernments, making more strict standards and lower MRL continuously, and so exports of chilli ofour country are facing more and more technical barriers. Thus, an efficient and selective method foranalyzing the residues of kasugamycin and carbendazim in chilli was set up and research onthe degradation dynamics of kasugamycin and carbendazim was carried out to evaluate theimpact of using kasugamycin and carbendazim in chilli field ecosystem, so as to reveal thepossible approaches of its degradation. This would help to provide basic information fordeveloping regulations to guard a safe use of kasugamycin and carbendazim in chilli fieldsand to prevent any health problem from consumers.1. We optimized the chromatogram condition, chose the suitable chromatographic column, mobilephase conditions and selected ions, and then successfully established a UPLC-MS/MS detectionmethod for the target. In this chromatogram condition, kasugamycin and carbendazim wereseparated well and correlation coefficients were greater than0.99.2. Analysis of residues of kasugamycin on chilli using SPE sample preparation and liquidchromatography mass spectrometry. Under the experimental conditions, the average recoveries ofkasugamycin were between77.82%and83.35%in the spiked level of8.0,40.0and120.0μg·kg-1in blank samples of chilli and soil, the coefficient of variation between2.20%and6.64%,the minimum detectable amount was5×10-12g, the minimum detectable concentration ofkasugamycin were2.5μg·kg-1in chilli and soil. The improved method basis NY/T-761wasused to extract, purify carbendazim in chilli samples, and analisis by liquid chromatographymass spectrometry. Under the experimental conditions, the average recoveries of carbendazim werebetween85.81%and88.60%in the spiked level of2.0,50.0and100.0μg·kg-1in blank samplesof chilli and soil, the coefficient of variation between2.31%and4.23%, the minimumdetectable amount was2.5×10-12g, the minimum detectable concentration of carbendazimwere1.0μg·kg-1in chilli and soil. It showed that the method can be used to study the residualbehavior of kasugamycin and carbendazim in the chilli fields. 3. The degradation test of50%kasugamycin and carbendazim WP （the kasugamycin4%,carbendazim46%） was carried out chilli fields at Shandong Jinan, Zhejiang Hangzhou andHebei Shijiazhuang in2009and2010. The results showed that the degradation ofkasugamycin and carbendazim followed an equation of one-grade chemical reaction dynamicsin the chilli and soils. The degradation half-life of kasugamycin in ranged from2.15d to3.52din chilli, and the degradation half-life of kasugamycin in ranged from2.40d to3.05d in soil.The degradation half-life of carbendazim in ranged from4.90d to6.40d in chilli, and thedegradation half-life of carbendazim in ranged from4.39d to5.55d in soil.4.50%kasugamycin and carbendazim WP （the kasugamycin4%, carbendazim46%） ofreasonable guidelines for the use of chilli was developed.50%kasugamycin and carbendazimWP was used to control chilli anthracnose,1410a.i.g/mu （the concentration of activeingredient: kasugamycin is56.4a.i.g/mu and carbendazim is648.6a.i.g/mu） was apply,sprayed2times, the safe interval of14d. The final residue of kasugamycin was lower40μg·kg-1and carbendazim was lower0.1mg·kg-1. |
PDF Full Text Request