Cloning And Analysis Of Resistant Functions Of Transcirption Factor ZmWRKY25From Zea Mays

Plant was always exposed to a variety of adverse envirment in life of which seriousharmful to plant growth, development, yield and quality, and even lead todeath.Thus,plant generated series mechanisms to resist these damage and theseregulations to various stress stimuli are always not a single pathway but alwaysmulti-pathways,coordinated with multiple genes from different levels response tostresses. The regulations in transcription level are more and more important, and now theWRKY transcription factor has become one of the key point in the gene function research.WRKY transcription factor involved not only in plant growth and development,metabolic control, and it is also involved in the regulation to the process of plants tobiotic stress and abiotic stress.The following progresses were gain in this study:This study successfully cloned a WRKY gene by PCR method,take the maize inbredline B73cDNA library as template, use the primers designed according to the sequencepublished at GenBank, Accession No. is NM₀01158417.The nucleotide sequence totallength is936bp, encoding311amino acids of the coding region, suggesting the relativemolecular mass is31.88kD, isoelectric point is9.89, containing the transmembraneregion of the protein according to bioinformatics softwares.ZmWRKY25protein is closerwith OsWRKY25from rice in the genetic distance of homology analysied with thephylogenetic tree constructed. This protein encoding signal peptide and nuclear exportsignal with online bioinformatics, and subcellular localization prediction nucleus. Thestudy successfully obtained some T3generation of transgenic Arabidopsis plants with themethod of agrobacterium tumefaciens-mediated transformation use the strainEHA105,contianing the vector pBI21∷WRKY25constructed, it has throught screeningunder50mg L-1kanamycin and PCR detected. The expression of ZmWRKY25gene can be rapidly induced by drought stresssimulated by PEG6000in the corn.Overexpression ZmWRKY25gene in Arabidopsis mayincrease its tolerance to drought tolerance.The ZmWRKY25gene can be rapidly induced by added exogenous ABA in the corn.ZmWRKY25may be relyed on and play positive roles in ABA-mediated signalingpathways.In summary, ZmWRKY25gene was studied in this paper,which is one of the WRKYtranscription factors.However, its downstream genes regulated by ZmWRKY25did not dofurther exploration, as well as its roles in the signaling pathways have not studied at themolecular level,so,this experiment can be perfect in these several aspects for the study ofregulation mechanism of WRKY transcription factors involved in plant stress andimproved the resistance of corn.