The Construction Of Genetic Map And QTL Mapping Of Some Energy Related Traits In Sweet Sorghum

Searching for renewable feedstocks for bioenergy to subsidize fuel shortage, it has beenrecognized that sweet sorghum is a viable candidate for bioethanol production because it is highsugar content, high photosynthetic efficiency and resistance to the barren. However, sweetsorghum breeding, cultivars, and varieties suitable for bioethanol production are essentiallylacking at the present time. In order to become a high-performance energy feedstock, biofuelsweet sorghum crops requires higher sugar yield and breeding high-quality varieties. Like otherenergy crops, become one of the important bioenergy crops in the future. To meet the demandfrom sorghum growers and the bioenergy industry, we will through conventional plant breedingand molecular marker-assisted selection technology to identify biofuel-related traits or genes. Inthis study, we build the SSR molecular marker linkage map with sweet sorghum PI653411,LS-A generation plants bagged self obtained287F₂plants, and energy-related traits includingplant height, stem diameter,biomass and brix in stem juice were quantitative trait locus（QTL）mapped. in order to providing foundation for marker assisted selection in sweet sorghumbreeding and precise mapping of major effect QTL.Establishment of SSR reaction system. Of330SSR primer pairs,34primer pairs had thepolymorphism between parents（PI653411x LS-A）. The polymorphic ratio was10.3%.Combination of the SSR-PCR amplification results and statistics of the phenotypic, Followingmain research results were obtained:1. SSR-PCR among the F₂260progenies and parents using34polymorphic primer pairs.Combination of phenotypic and genotypic data, Sorghum genetic linkage map and linkageanalysis were performed with MapMaker3.0using Kosambi which converts recombination rateto cM.17markers distributed in10linkage groups of sorghum from Chromosome c01a,Chromosome c01b, Chromosome c02, Chromosome c05, Chromosome c09, Chromosome c10a,Chromosome c10b, covering a total distance of147.1cM.2. The QTLs in single marker analysis, IM, CIM and MIM were found by means ofWindows QTL Cartographer2.5. A main effect QTL on linkage groups of sorghum on the10th,four primers mark the PepC, Xtxp127, and Xtxp101, Xtxp06control of sweet sorghum biomass,stem diameter, plant height of energy traits.