Quality Evaluation, Cultivation And Hairy Roots Culture Of Wild Polygala Speicies From Lao Mountain In Qingdao City, Shandong Province, China

Radix Polygalae was one of 40 kinds of commonly used Chinese herbal medicines in China, which was collected from wild plants of Polygala genus. However, the plant resources of Polygala has become endangered in recent years because of excessive collection, and the cultivating technology of Polygala which has been testing have some problems, such as decreasing quality and smaller yields. Here, in order to protect the wild resources, solve the problem of supply and exploit the important compounds of Polygala, wild plant resources of Polygala in Lao Mountain, Shandong province of China were investigated, their active constituents were determined, and the best species were chosen for cultivation and hairy roots culture.Resources:Four wild species belong to Polygala genus, P. tenufolia Willd., P. sibirica L., P. japonica Houtt., and P. tatarinowii Regel, were found in Lao Mountain. Among the four wild species, the quality of P. tenufolia and P. sibirica measured up to the standards of Ch.P (2010) accoding to the contents of saponins,3, 6’-di-O-sinapoyl-sucrose and xanthone III in their roots, and the quality of P. tenuifolia was better than P. sibirica.Breeding method of P. tenuifolia:(1) 500 mg/L GA3 and 25% PEG promoted seed germination of P. tenuifolia significantly, and could be used in seed priming for cultivation; (2) 200 mg/L NAA promoted the rooting of cutting shoots of P. tenuifolia, and also could be used in cultivation.Cultivation of P. tenuifolia:(1) Water, moderate water stress treatment could promote the accumulation of the main active ingredients and increase the yield of P. tenuifolia; (2) Fertilizer, phosphorus fertilizer could improve the content of the main active ingredients of P. tenuifolia; (3) Growth years, the roots of P. tenuifolia should be collected after 3 years in view of yield and quality; (4) Harvest time, the middle of May was the best time for harvest.Hairy roots culture of P. tenuifolia:Hairy roots were all induced from the hypocotyl explants of P. tenuifolia by Agrobacterum rhizogenes A4, R1600 and R1000, and the content of 3,6’-di-O-sinapoyl-sucrose was higher in the hairy roots induced with A. rhizogens A4 than the two others.