Research About Distribution Of Variations In Inron3 Of Agouti Gene In Different Coat Color Goat

The primers are designed according to the sheep Agouti gene(EU185099) that has published in GenBank. The sequenced length of 3’UTR region of goat is 190bp. The g.A4756C、g.C4971T、g.A5344G and g.AGG5124-5125 variable sizes are found in the 3rd intron of goat Agouti gene, and then analyse the four variation sites. Research shows that they have six haplotypes that is ACAA(71.31%)、ATBG(13.11%)、CTBG(7.38%)、ATAA(4.92%)、ATAG(1.64%)and CTAG (0.82%) in the four variable sites. Almost all individuals of JN with grey coat color, LH with black coat color and LR with white coat color were haplotype ACAA, but NK with brown coat color had different haplotypes. It is infered that these variable sites may be play a certain role in color goat of the process of forming.A and C alleles of g.A4756C locus were found in the 3rd intron of goat Agouti gene, and the frequency of A allele is high in cinerous, white, and black variey. The frequency of A allele is 54.69% in Brown goats that the distribution of three genotype is hypodispersion. The C allele of g.A4756C may exist chain relation with sites that controlled the brown phenotype of goats, and CC genotype is related with brown hair by the analysis.The C and T allele of g.C4971T locus were found in intron region of Agouti gene. The frequency of C allele is 98.33% in JN, the frequency of C allele is 93.33% in LH, the frequency of C allele is 100% in LR and the NK, which the genotype is all TT. The T allele of g.C4971T may exist chain relation with sites that controlled the brown phenotype of goats, and TT genotype is related with brown hair by the analysis.The A and G allele of g.A5344G locus were found in the 3rd intron of Agouti gene. The A allele was dominated in JN、LH、LR, The genotype was AA in JN and LR, and AA genotype is absolute advantage in LH, on the contrary, the GG genotype was absolute advantage in the NK. The G allele of g.A5344G may exist chain relation with sites that controlled the brown phenotype of goats, and GG genotype is related with brown hair by the analysis.The A and B allele of g.AGG5124-5125 insert locus were found in the 3rd intron of Agouti gene, which the A was not inserted the AGG, but B was inserted by AGG. The A allele was dominated in JN, LH and LR, the frequency of A allele was 98.33%, 90.00% and 96.67% separately. The genotype was AA in JN, the genotype was AB in LR, and three of genotypes were existed in LH, the AA genotype was rich in these three breeds, on the contrary, the BB genotype (71.87%) was absolute advantage in the NK. So we conjectured that the B allele of g.A5344G may exist chain relation with sites that controlled the brown phenotype of goats, and BB genotype is related with brown hair by the analysis. After the single times type analysis,found six single times type that ACAA(71.31%)、ATBG(13.11%)、CTBG(7.38%)、ATAA(4.92%)、ATAG(1.64%)、CTAG(0.82%). Almost the JN and LR is ACAA, the single times type that ACAA is small proportion. But there are different single times type in LH and NK, single times type that CTBG could relate with brown phenotype.The result of cluster shows that the 4 local breeds of goat were divided into two classes, the one is the NK that allele has the brown hair phenotype; the other one is JN, LH and LR, which allele has black, grey and white hair phenotype. The relative analysis shows that the mutations of A4756C、g.C4971T、g.A5344G and g.AGG5124-5125 locus were significantly related with hair of goat in these 4 local breeds. The result of cluster can just reflect the heredity relation of these 4 local breed goat by the A4756C、g.C4971T、g.A5344G and g.AGG5124-5125 locus of Agouti gene, but not real heredity relation. We need to further study in order to the genetic relationship of 4 local goat breeds.