The Development Of ICT-based Reactive Fluorescent Sensors

With the rapid development of technology and urgent needs of biological research, fluoremetric method has been widely used due to its low-cost, easy of operation and real-time detection. Fluorescent probes based on mild chemical reactions has been drawn an increasing attention because of some superiorities like high selectivity and sensitivity.Hydrazine (N2H4) as well as its derivatives is not only an important industrial raw material but also a kind of neurotoxin and carcinogen. Therefore, the accurate detection of trace hydrazine in water and biological samples will be of importance to biological and medical sciences. Utilizing N-coumarin compound, we have successfully designed a novel hydrazine fluorescent probe (1-CN) which has a long absorption and emission wavelength. This probe could realize colormetric and ratiometric determination of hydrazine through reactions with hydrazine. The sensitivity of probe1-CN towards hydrazine is very high with a detection limit of0.70nM. Besides, this probe enables rapid response time for hydrazine determination which is important for real-time detection. What’s more, this probe could realize detection for intracellular hydrazine which will be a useful tool for studying biological processes involving hydrazine within living cells. This is the first ratiometric fluorescent probe for hydrazine that is expected to be utilized in a variety of chemical and biological applications.Hydrogen sulfide (H2S) is now considered as an important gasotransmitter which involves in various physiological processes. Accurate and reliable measurement of H2S concentrations in vivo will provide useful information to study the function of H2S deeply. In order to enrich the types of the probe and realize in vivo imaging, a kind of fluorescent dye with two-photon absorption1-NH2has been successfully designed which has a NIR emission in several kinds of solvent. Based on1-NH2, we further developed a H2S probe1-N3which has been used to realize H2S specifically. Probe1-N3has a good selectivity towards H2S determination with no interference from reactive oxygen species, sulfide species and other reducing reagents. This probe could realize H2S detection in fetal bovine serum using a low concentration. Utilizing the two-photon property,1-N3has been used to imaging H2S in living cells and tissues. Besides, this is the first successful fluorescent probe for H2S detection in living mice.