Study On Extraction And Purification Of Tea Saponin And Protein From Pomace Of Camellia Oleifera Abel

This subject is based on the Camellia oleifera seed cake as the raw material. Wemainly study the best technology of the extraction, separation, and purification of the teasaponin in the Camellia oleifera seed cake and the determination method of the tea saponin.And we makes a study of the extraction, separation and the function characteristics of theprotein in the Camellia oleifera seed cake. It provides a theoretical basis for the industrialproduction of the tea saponin. And it has important significance to improve thecomprehensive resource utilization.1. Main nutrients in the Camellia oleifera seed cake: Total sugar35.19%, fat6.19%,protein8.12%, crude fiber18.67%, tea saponin12.11%.2. Making a qualitative experiment of the extracts of the Camellia oleifera seed cakethrough TLC, it finds that it appeared the same spots at the same Rfvalue position in thetea saponin reference substance of the extracts.3. Use methanol as the extraction agents to extract the tea saponin in the Camelliaoleifera seed cake bread by ultrasonic auxiliary method. Take the General rotatorycomposed test to investigate the tea saponin extraction efficiency. The best extractiontechnology is than1:9of the liquid material. And the best technological conditions of theextraction of the tea saponin by ultrasonic auxiliary method are the ultrasonic temperaturefor50℃, and the ultrasonic extraction time for57mins. In these conditions, the teasaponin extraction efficiency can reach80.89%.4. Establish the High Performance Capillary Electrophoresis method, the conditionsfor detcting the tea saponin are: the BECKMAN High Performance CapillaryElectrophoresis apparatus, the PDA detector, the not coating melted quartz capillarycolumn (i.d.50um x60cm, effective length50cm),30mM boric acid borax solution asthe running buffer a (pH=8.0), the injection time for5s, the capillary column temperaturefor25℃, the separate electrophoresis for20kv, and the detected wavelength voltage for210nm. Then compared with the vanillin strong sulfuric acid by the spectrophotometricmethod, the results shows that the High Performance Capillary Electrophoresis method isnot easily affected by other impurities, and its precision is high.5. Use macroporous resin and polyamide resin to purify the tea saponin respectively,and investigate the elution agents concentration of the two methods with70%and50%ethanol respectively. The objective content purity of the two purification methods is 83.23%,80.17%.6. Use alkali mention acid sinking method to extract the protein in the camellia bread,and take the orthogonal test to investigate the protein extraction. The best extractiontechnology is: extraction temperature for45℃, pH value for11.5, and the90mins’extraction of the material liquid under the condition of than1:45. In these conditions, theprotein extraction rate is44.19%.7. Measure the function characteristics of the camellia protein. The result is: theoil absorbing for4.0ml/g, the emulsification for36%, the emulsion stability for55%, andthe foaming ability for60%.