Study On The Process Of Swill Oil Transformed Into Biodiesel Catalyzed By Molecular Sieve Immobilized Lipase

The soaring consumption of petrochemical resource and the severe global energy complexion have become one of the main drives for the constantly increasing petroleum price in recent years. Biodiesel, a new green fuel alternative, holds such merits as environmental benefits and sustainability or reproducibility. The thesis adopts Mesoporous Molecular Sieve, with MCM-41being the carrier. The experiment applies the physical absorption to immobilize the neutral lipase, which will be used to catalyze the hogwash fat to generate biodiesel. The experiment proceeds with the study on the application amount of the lipase, immobilizing duration and the temperature, and their impacts on the activity of the immobilized lipase. Spontaneously, the study is focusing attention on the interactivity of such influencing factors as the alcohol oils’molar ratio, reaction temperature, time and the compensation of normal hexane.Mesoporous molecular sieve as the carrier,when the lipase quantity for45960U·g-1, temperature45℃, pH7.5, reaction time3h, the activity of immobilized lipase was approximately4666U·g-1. Temperature stability and PH stability of the immobilized lipase have improved obviously than the free enzyme,and the optimum reactive pH of immobilized lipase change from8to7.5, the most suitable temperature does not change obviously. The immobilized enzyme particles which entrapped sodium alginate could utilized for five times, the relative activity was below20%, but then the particle was easy disintegration.A self-designed circulation reactor was developed to carry out the transesterification between waste oil and methanol on immobilized lipase catalyst. The single factor experiments to determine the initial optimization of reaction conditions:The molar ratio of methanol to oil3:1, temperature45℃, reaction time9h, hexane27g. Used response surface to interactive experiments, determined the optimized conditions:The molar ratio of methanol to oil3.16:1, temperature44.06℃, reaction time8.07h, conversion rate61.0242%. Model was noticeable.