Study On Spearmary Cells Apoptosis Induced By Tbtcl In Oreochromis Aureus

Tributyltin chloride (TBTCL) is the important one of Organotin Compounds, and it has the heaviest toxicity and widest application in them. And up to now, it is the most heavily toxic in all chemicals discharged into the sea. And it is mainly used to be marine antifouling coating, agricultural insecticide, and polyvinylchloride(PVC) stabilizer et al. So wide application makes TBT have much possibility to go into the water environment. Yang Ruiqiang have detected TBT in fish body in the Meiliang Bay region and the East Taihu lake region, and other scientists have detected TBT in many water areas, which indicate that we have to have much knowledge about the toxicity characteristic of TBT. and about the threat brought into the aquatic animals and human beings. So far, some datas show that TBT can make sex distortion happen in some mollusca species, can damage genomic DNA of some species of mullusca and fish, and can also damage the antioxidant defense system of some species of mollusca and fish, and even can make some cell types apoptosis. And of all the cell types, we have not found the germ cell.Taking the male Oreochromis aureus as experiment material, studies on spermary cells apoptosis in fish induced by tributyltin chloride (TBTCL) were carried out under the method of terminal deoxynucleotidy transferase mediated dUTP-Biotin nick-end labeling (TUNTEL), and at the same time, we detected changes of the activity of superoxide dismutase (SOD) and the content of Glutathione (GSH) in the liver and spermary after TBT was intraperitoneally injected into Oreochromis aureus, so as to know wether TBT can damage the antioxidant defense system of Oreochromis aureus. And also, we detected the change of the activity of Ca2+-ATP enzyme after TBT was intraperitoneally injected into Oreochromis aureus and after the Oreochromis aureus were expsured in TBT. All the experiments above were to test whether TBT can cause apoptosis of spermary cell of Oreochromis aureus, whether it can cause the change of activity of Ca2+-ATP enzyme, and the relationship between the apoptosis and activity of Ca2+-ATP enzyme, and the relationship between the apoptosis and the activity of SOD and the content of GSH. The results showed that:under the exposure way of injection,24hours and48hours after contamination, along with the dosage elevating, the spermary cell apoptosis rate of the tilapia increased and then reduced, and when the dosage was5μg/kg body weight,the apoptosis rate reach the highest;96hours after the contamination, the cell apoptosis rate reduced and then increased,and when the dosage was5μg/kg body weight the apoptosis rate reach the lowest. The results of the Ca2+-ATP enzyme of spermary cell show that the difference of the low monitoring team (1-3μg/kg) and the control group was not remarkable; under the exposure way of putting the fish into different level of contamination waters, along with the dosage elevating, the spermary cell apoptosis rate of the tilapia increased. The results of the Ca2+-ATP enzyme of spermary cell show that it can be induced to a relatively high level by TBTCL,and with the dosage elevating,the activity of Ca2+-ATP enzyme increased.At the dosages of the experiment,the activity of Ca2+-ATP enzyme increased in Coordination with the apoptosis rate of spermary cells of the male Oreochromis aureus.The possible reason for this is that Ca+-ATP enzyme was induced in some way by TBTCL,and then the thickness of Ca2+in cytoplasm increased,which can be a factor of the appear of apoptosis.To sum up, TBT can cause apoptosis of spermary cells of Oreochromis aureus, and the happen of apoptosis has something to do with the change of the activity of Ca2+-ATP enzyme, and may be related to the damage of antioxidant defense system of liver and spermary.