Study On Lignans And Esters Of Acanthopanax Senticosus

Acanthopanax Senticosus (Ruper. et Maxim.)Harms are the plant of family Araliaceae. The Acanthopanax Senticosus (Ruper. et Maxim.)Harms are widely used as the common Chinese herbal medicine in the folk. Its roots and rhizomes have been widely used for the treatment of neurasthenia, cardiovascular disease, Parkinson’s disease, arteriosclerosis, diabetes, rheumatism, and also used for anti-fatigue, and improve body flexibility and treatment cancer adjuvant drugs.This paper first outline the research progress of chemical constituents and pharmacological of Acanthopanax Senticosus (Ruper. et Maxim.) Harms in recent years. Studying on Acanthopanax Senticosus (Ruper. et Maxim.)Harms has been the research focus of Natural Medicinal Chemistry in recent years, by the two aspects of the pharmacy and clinical to study the Acanthopanax Senticosus (Ruper. et Maxim.)Harms, and found that Acanthopanax has more pharmacological activity and monomer compounds with pharmacological activity were isolated. However, both the Tyrosinase inhibition activity and free radical scavenging activity of the Acanthopanax Senticosus (Ruper. et Maxim.)Harms have little been reported in the literature. We first do the Tyrosinase inhibitory activity and DPPH radical scavenging activity experiment for the different fraction of the extracts and components of the Acanthopanax Senticosus (Ruper. et Maxim.) Harms. According to the results of the Tyrosinase inhibitory activity and DPPH radical scavenging activity, we choose the better activity fraction of the extracts for further separation and purification, and then we do the Tyrosinase inhibitory activity and DPPH radical scavenging activity test for the monomer compounds, and calculate their IC50values.In this experiment, the Tyrosinase inhibitory activity and DPPH radical scavenging activity are used as a guide. The silica gel column chromatography ODS reversed phase column chromatography, macro porous resin, Sephadex column chromatography, PTLC, and semi-preparative HPLC and other separation methods were used to isolate the ethyl acetate extract of the Acanthopanax Senticosus (Ruper. et Maxim.)Harms, and21compounds were isolated, the property of physical, chemical and modern spectral analysis techniques (UV, IR, NMR and MS and so on) was used to establish the structures of fourteen compounds. erythro-Guaiacylglycerol-β-O-4’-dehydrodisinapyl ether (1), Syringaresinol (2), Lariciresinol (3), Tripterygiol (4), Diisobutyl phthalate (5),[Bis (2-ethylhexyl) phthalate,6],3,3’-dimethoxy-4,8’-oxyneoligna-9,4’,7’,9’-tetraol (7), threo-Guaiacylglycerol-/β-O-4’-sinapyl ether (8),8,8’-bisdihydrosiringenin (9),1-(4’-Hydroxy-3’-methoxy-phenyl)-2-[4"-(3-hydroxypropyl)-2",6"-dimethoxyphen oxy]propane-1,3-diol (10), Isofraxidin (11), Savinin (12), Balanophonin (13), sesamin (14). Except the compounds2and11, other compounds were first isolated from Acanthopanax. The results of the radical scavenging activity towards DPPH test showed that compounds2,3,4and11have better activity. The IC50values were0.66,0.01,0.01and0.01μg/mL, while the positive reference vitamin C with an IC50value of2.58μg/mL, so the antioxidant capacity of the compounds2,3,4and11than vitamin C. The active compounds were isolated in this experiment will provide the basis of experimental evidence for the further development of Acanthopanax...