Study On Microbial Community Succession And The Influence Of Adscititious Species To Pyrite Bioleaching

Bio-leaching is gradually paid attention to because of its less energy consumption low costing and environmentally-friendly. In the Hydrometallurgy area, many researches focus on using single species leach minerals less on the mixture bacterium, so further research on the mixture bacterium can be theoretical and practical meaningful to the leaching rate.Firstly, the dissertation focus on using the Real-Time PCR to monitor the community structure and succession, and integrate the leaching rate of the pyrite in order to discuss the relationship between community structure and the function. We find that in the8species mixed system, At. caldus S1became the dominating specie during the prophase which reach99.75%; L. ferriphilum LY became the dominating specie during the anaphase which reach73.63%, both of them can accelerate the leaching rate of the pyrite. And at the anaphase Ferroplasma L1was gradually became the third dominating specie which occupys10.97%. The results indicate L. ferriphilum LY play an important role in the pyrite bio-leaching, the leaching rate increase with the proportion of the L. ferriphilum LY in the mixed system.Secondly, the dissertation takes At. caldus S1, L. ferriphilum LY as the research object which can be aboriginal specie or adscititious specie, discuss the influence on the by adscititious specie during the different phase (lag phase exponential prophase exponential metaphase). The results show that:adding the adscitious specie at the exponential metaphase can great accelerate the leaching rate of the pyrite, the latter is at the lag phase, then the exponential prophase. At the exponential metaphase, taking the At.caldus S1as the aboriginal species has much contribute than L. ferriphilum LY, it needs14days to leach pyrite completely. The results also show that the quantity of the L. ferriphilum LY increasing can arouse the increase of the leaching rate, but the At. caldus S1cannot.